The invention aims at providing a scheme capable of reversing the medicine resistance fundamentally, improving the curative effects of chemotherapy medicines and reducing the toxic and side effects ofthe chemotherapy medicines, and particularly provides application of grape seed procyanidine in preparation of a combination medicine in a cancer chemotherapy scheme. The combination medicine is usedfor being cooperatively used with the chemotherapy medicines used for treating cancers, and therefore the phenomenon is reduced that due to the medicine resistance exists, the effects of the chemotherapy medicines are reduced, or the risk of toxic and side effects occurring when the dosage of the chemotherapy medicines is increased for reducing the medicine resistance is reduced.