The invention relates to a vaccine vector preventing the FAdV-4 and the NDV and a preparation method and application of the vaccine vector, and belongs to the technical field of prevention for the FAdV-4 and the NDV. According to vaccine vector and the preparation method and application of the vaccine vector, based on the structure characteristic of the FAdV-4, an FAdV-4 protective antigen Fiber 1protein is selected as a research and development object, and by constructing a recombinant Newcastle disease virus expressing the Fiber 1, a novel genetic engineering vaccine capable of preventing the FAdV-4 and the NDV is obtained through research and development; through research and development of the vaccine, not only is an important tool provided for prevention and control over the FAdV-4,but also a LaSota toxic strain of the Newcastle disease virus is selected as the vaccine vector, the toxic strain is a common vaccine toxic strain in production, an effect can be achieved that one strain prevents the two viruses, and therefore the vaccine immunization process is simplified.