The invention discloses a display substrate and a manufacturing method thereof, and a display device, and relates to the field of display technology. The following problem is solved: when the TVG technology is applied to a backboard, a conductive material located in a substrate through hole of the backboard is prone to swelling, such that the coupling between functional patterns located on upper and lower surfaces of the substrate is broken, which reduces the production yield of the backboard. The manufacturing method of the display substrate comprises the steps of: manufacturing a first functional structure on a first side of a common substrate, and manufacturing a second functional structure on a second side of the common substrate; making a through hole in an edge area of the common substrate; and manufacturing a conductive connection portion in the through hole, wherein a first end of the conductive connection portion located on a first end of the first side extends out from the through hole and is coupled with a first functional pattern in the first functional structure, and a second end of the conductive connection portion located on a second end of the second side extends out from the through hole and is coupled with a second functional pattern in the second functional structure. The manufacturing method disclosed by the invention is used for manufacturing the display substrate.