The invention discloses a processing and production method of strong-flavor pumpkin seed oil. Pumpkin seeds with shells are dried, then unshelled and subjected to kernel and shell separation, and pumpkin seed kernels are obtained; the pumpkin seed kernels are broken, and the broken pumpkin seed kernels are obtained; the pumpkin seed kernels are subjected to ultrasonic treatment and dried, and thepumpkin seed kernels subjected to ultrasonic treatment are obtained; the pumpkin seed kernels subjected to ultrasonic treatment are extracted, after extracting is completed, pumpkin seed oil and pumpkin seed cake dregs are obtained; the pumpkin seed cake dregs are crushed, pumpkin seed cake dreg powder is obtained, the pumpkin seed cake dreg powder and distilled water are mixed and stirred, a compound protease is added for enzymolysis, after enzymolysis is completed, enzyme deactivation treatment is conducted, then through centrifugal separation, supernate is taken, and then a pumpkin seed enzymatic hydrolysate is obtained; and the pumpkin seed enzymatic hydrolysate is evenly mixed with the pumpkin seed oil, a mixture is placed into a thermostatic oil bath pan to be subjected to a Maillardreaction, centrifugal filtration is conducted, and then the strong-flavor pumpkin seed oil is obtained. According to the processing and production method of the strong-flavor pumpkin seed oil, a process is safe, and preparation is convenient and rapid.