The present invention discloses a fusarium oxysporum strain and belongs to the technical field of pathogenic microorganism isolation. The fusarium oxysporum is fusium oxysporum Hs-f (Fusarium sp.), isdeposited on August 29, 2019 in China General Microbiological Culture Collection Center and has a deposit number of CGMCC NO:18130, the address is No.3, No.1 courtyard, Beichen West Road, Chaoyang District, Beijing, and a unit asking for deposition is Peanut Institute of Shandong province. The peanut rot pathogen fungus is isolated and purified from peanut rot disease seed kernels. Inoculation experiments prove that the fusarium oxysporum strain has high pathogenicity, is also free of tissue specificity, and has pathogenicity to peanut roots, stems, leaves and seed kernels.