The invention discloses a preparation method of flavor-enhanced low-fat-content crab-ovary fresh-shrimp sauce. The preparation method of the flavor-enhanced low-fat-content crab-ovary fresh-shrimp sauce comprises the following steps: (1) preparing a compound essential oil sustained-release agent; (2) preparing a fat substitute; (3) performing pretreatment on soybean sauce; (4) picking out crab ovary; (5) washing white prawns; (6) stir-frying sauce materials; and (7) carrying out filling and sterilizing. According to the preparation method of the flavor-enhanced low-fat-content crab-ovary fresh-shrimp sauce, the fat substitute is developed and applied in preparation of the crab-ovary fresh-shrimp sauce, so that, taste, texture state and flavor of the crab-ovary fresh-shrimp sauce are significantly improved.