The invention belongs to the technical field of heat conduction materials, and particularly relates to a method for preparing a heat conduction interface material. The method for preparing the heat conduction interface material includes the following steps that S1, raw materials are mixed; S2, orientation and alignment are performed; S3, after a heavy object is pressed on a container in the step S2, the container with the heavy object is put in a vacuum drying box which is vacuumized to be less than or equal to -0.09 MPa, and then after 5-10 minutes, vacuum release is performed; after removingthe heavy object, the container is put in the vacuum drying box which is vacuumized to be less than or equal to -0.09 MPa, after 5-10 minutes, vacuum release is performed, and then the materials arecompacted in a vibration compactor; and S4, curing is performed, and the heat conduction interface material is obtained.