The invention belongs to the technical field of metering test, and particularly relates to a LabVIEW and series communication-based sensor data acquisition method and system. By the LabVIEW and seriescommunication-based sensor data acquisition method and system, the problems that an existing displacement sensor calibration data acquisition method is long in time, low in efficiency, high in cost and long in development period and is easy to make mistakes are solved. Under the condition that an existing manual calibration device is not changed, data transmission acquisition with LabVIEW data acquisition software in a computer is achieved by a RS232 series port in a digital multimeter, linear accuracy calculation and uncertainty analysis are performed, various defects of manual data input are prevented, meanwhile, new equipment is not needed to be purchased, and the cost is reduced.