This invention discloses a kind of method using microorganism fermentation to prepare glycosides or glycosidic preparation from glycosidic matter of drugs. This method comprises the steps of: The glycosidic drugs must fibe crushed to pieces, and then mixed with water at a certain rate, after antisepticised under 115-121Deg. C for 10-20 minute. When this solution is cooling, it must be gave microorganism bacterium to make fermentation. It strips down the hepatin and leaves the glycoside matter. Lastly when the fermentation is stopped, we distill the production from the mixture with organic solvent and then purified them to be pure glycosides. The pure glycosides can be made to different drugs with the common way of impregnant subject, includes: pieces, capsule, injection and so on. This invention conquered the shortcoming of traditional way, which can make the structure and conformation changed, and simplifies the process of prepare and transform, enhances the efficiency of transform and dissolution. The prepared reagent has a quick absorption, a low dosage and toxicity, quick affection, high usage efficiency.