The invention provides a device and a method for transferring a glass substrate. The device comprises: an air supplying means for supplying air; a spraying plate having a plurality of ejecting holes, through which air is ejected; and a shield plate for partly blocking air ejected from plurality of ejecting holes. According to the invention, the pollution from power grains to the glass substrate can be prevented, dut to the glass substrate is transferred without physically contacting with any device, such as roller, etc. In addition, gas suspendes the glass substrate in the air continuously, as a result, the glass substrate will not tilt during transferring period.