The utility model discloses an immunofluorescence chromatography test strip for detection of Miltenberger antibody. The test strip comprises a sample pad, a reaction membrane and an absorbent pad which are sequentially lapped on a lining plate, wherein two ends of the sample pad are respectively provided with a first sample adding point and a second sample adding point, an inner quality control belt and at least an antibody detection belt are fixed on the reaction membrane. By adopting the immunofluorescence chromatography test strip, the problem that simple and practical conventional clinical Miltenberger blood group antibody detection technologies and reagents are lack in China or even in the world is solved, the test strip can be used for assisting in diagnosing diseases such as hemolytic disease of the newborn and adverse effect of blood transfusion caused by Miltenberger blood group antibody in clinic, and guarantees safe, effective and scientific blood transfusion in clinic.