The utility model relates to a film shearer for filamentous fungus genetic transformation of agrobacterium-mediated transformation. The film shearer is particularly applied to the filamentous fungus genetic transformation of the agrobacterium-mediated transformation. The film shearer comprises a handheld part and a cutter bit, wherein the cutter bit comprises a triangular formed by a front edge, a rear edge and a blade; the included angle between the front edge and the blade is 40-50 degrees, and the included angle between the rear edge and the blade is 25-35 degrees; the handheld part is connected with the rear edge of the cutter bit and is parallel to the front edge of the cutter bit. The film shearer can be operated by a single person, has a uniform shearing function, and is wide in application, the labor intensity is greatly alleviated, and the transformation efficiency is greatly improved.