The utility model provides a hinder fungus nature test device, relates to wound dressing's the fungus nature that hinders research techniques field, and its structure is including being used for holding the challenge room of challenge inoculum and the sampling room that is used for cleaning the sampling, and challenge room and sampling room are put together and are constituted the test device main part, be provided with the sample article that supply between challenge room and the sampling room, it is tight that challenge room and sampling room will supply to try the specimen holder, being equipped with on the challenge room and challenging the room mouth, be equipped with sampling room mouth on the sampling room, challenge room mouth all is equipped with the jam with the room mouth department that samples. The utility model discloses a test device can to claim have the wound dressing that hinders the fungus performance hinder fungus performance evaluation, and covered the different states of dressing, the evaluation result science is comprehensive.