The utility model discloses an air regulation system of application paint spray booth is provided, include: air conditioner air feeder group, exhaust unit, control unit, the power -driven air valve that supplies gas, exhaust power -driven air valve and amount of wind wind speed detection device. Air conditioner air feeder group links to each other through the top of supply gas tuber pipe and paint spray booth, and the exhaust unit links to each other through the bottom of exhaust tuber pipe with the paint spray booth, and the power -driven air valve that supplies gas sets up on the pipeline of tuber pipe of supplying gas, and exhaust power -driven air valve sets up on power -driven air valve's the pipeline that exhausts. Control unit is used for controlling the fan operation amount of wind of air conditioner air feeder group and exhaust unit, still is used for adjusting supplying gas power -driven air valve and adjusting the amount of wind with exhaust power -driven air valve's aperture. Amount of wind wind speed detection device is used for detecting each regional wind speed and the amount of wind in the paint spray booth, and control unit is according to the amount of wind of each regional wind speed and air regulation paint spray booth. The utility model provides an each unbalanced problem of the regional amount of wind in the paint spray booth improves the vehicle paint spraying quality and improves the operation environment.