The utility model discloses a glass culture dish that can automatic punch, including culture dish upper cover A, culture dish upper cover B, box at the bottom of the culture dish, the nail that punches constitutes. Culture dish upper cover A top inboardly have 9 sunken nail holes, it is threaded to follow closely downthehole wall, the nail top of punching there is the pin fin, the pin fin outer wall is threaded, and pin fin height and nail hole depth face with, the pin fin just can be twisted and gone into to follow closely the hole to a combines closely with the culture dish upper cover. Punch the nail height degree of nail for box at the bottom of culture dish upper cover A and the culture dish closes the back, the nail bottom of punching is 1.8~2.5mm's length apart from end box bottom portion.