The utility model discloses a biological indicator detection device for monitoring pipe chamber class mechanical sterilization effect, include: micropore length coiled tube chamber lid, linker and the biological cavity of instructing, be provided with coiled tube chamber passageway in the lid of micropore length coiled tube chamber, micropore length coiled tube covers in the chamber and is provided with first inlet port, second inlet port and venthole, connect on the venthole the linker, be provided with software sealing stopper, degerming filter membrane, culture medium bag and biological indicator carrier in the biological instruction cavity. The utility model is used for simulation lumen class apparatus, the actual working length of its coiled tube chamber passageway can adjust, makes lumen access structure more close with the lumen class apparatus of treating the sterilization, can improve the reliability of sterilization testing result, just the utility model discloses can effectively avoid outside microorganisms such as bacterium to get into the biological cavity of instructing, disturb the testing result, the utility model discloses small, use conveniently, have very high popularization and application and worth.