The utility model discloses a swirl blender test tube fixing device, including support and test tube cover, the support includes the base, is fixed with the montant on the base, is provided with the horizontal pole on the montant, horizontal pole and montant swing joint, the test tube cover includes slider and rubber sack, and the rubber sack is cylindricly, and seal its upper end, and the rubbersack is connected with the slider through the elastic rod, slider movable mounting is on the horizontal pole. Adopt the swirl blender test tube fixing device of above -mentioned structure, can effectively solve the easy spill of in vitro liquid, test tube easy broken, at every turn can only be to the technical problem that a test tube mixes and the support position can not be adjusted.