The utility model discloses a protein immobilization modified cell culture container which comprises a body and a cover, and the inner wall of the body is coated with a protein layer. When the cell culture container is used for culturing cells, protein does not need to be supplemented, repeated protein addition in the cell culture process can be avoided only through one-time protein coating, operation is greatly simplified, and the cell culture container is convenient, rapid, economical and practical; meanwhile, instability of protein mass in multiple preparation processes is avoided, so thatuncontrollable factors in the whole culture process are reduced, and the cell culture efficiency and quality are improved.