发明公开
EP0100217A2 Process for quantitative determination of substrate treated with oxidase
失效
维他命Zur定量Bestimmung eines mit氧化酶behandelten基材。
- 专利标题： Process for quantitative determination of substrate treated with oxidase
- 专利标题（中）： 维他命Zur定量Bestimmung eines mit氧化酶behandelten基材。
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申请号： EP83304262.5申请日： 1983-07-22
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公开(公告)号： EP0100217A2公开(公告)日： 1984-02-08
- 发明人: Sakata, Yoshitsugu , Miyashita, Yoshinobu , Hamanaka, Tadashi , Kodera, Hiroyuki , Miki, Yutaka , Yamanishi, Kazuhiko , Hanada, Toshiro
- 申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.
- 申请人地址： 10, Doshomachi-3-chome Higashi-ku Osaka JP
- 专利权人： WAKO PURE CHEMICAL INDUSTRIES, LTD.
- 当前专利权人： WAKO PURE CHEMICAL INDUSTRIES, LTD.
- 当前专利权人地址： 10, Doshomachi-3-chome Higashi-ku Osaka JP
- 代理机构： Baillie, Iain Cameron
- 优先权： JP128700/82 19820723; JP170639/82 19820929; JP110423/83 19830620
- 主分类号： C12Q1/26
- IPC分类号： C12Q1/26
摘要：
A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent. Further, the peroxidase can be replaced by (b') iron complex of porphyrin or ion chelate of complexane.
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