发明授权
- 专利标题： SECRETORY LEADER SEQUENCES
- 专利标题（中）： 分泌前导序列
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申请号： EP89909015.3申请日： 1989-07-14
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公开(公告)号： EP0387319B1公开(公告)日： 1996-03-06
- 发明人: GOODEY, Andrew, Robert , BELFIELD, Graham, Paul , SLEEP, Darrell
- 申请人： Delta Biotechnology Limited
- 申请人地址： Castle Court, Castle Boulevard Nottingham NG7 1FD GB
- 专利权人： Delta Biotechnology Limited
- 当前专利权人： Delta Biotechnology Limited
- 当前专利权人地址： Castle Court, Castle Boulevard Nottingham NG7 1FD GB
- 代理机构： Bassett, Richard Simon
- 优先权： GB8817598 19880723; GB8906920 19890328
- 国际公布： WO9001063 19900208
- 主分类号： C12N15/81
- IPC分类号： C12N15/81
摘要：
Secretory leader sequences, for use in secreting heterologous polypeptides in yeast, are formed by fusing part of the human serum albumin pre-sequence or part of the Kluyveromyces lactis killer toxin pre-sequence to the Saccharomyces cerevisiae mating factor alpha-1 KEX2 cleavage recognition site. The resulting fusion leader sequences are: (a) H2N-Met-Lys-Trp-Val-Ser-Phe-Ile-Ser-Leu-Leu-Phe-Leu-Phe-Ser-Ser-Ala-Tyr-Ser-Arg-Ser-Leu-Asp-Lys-Arg-COOH or (b) H2N-Met-Asn-Ile-Phe-Tyr-Ile-Phe-Leu-Phe-Leu-Leu-Ser-Phe-Val-Gln-Gly-Ser-Leu-Asp-Lys-Arg-COOH. Conservative variations are also encompassed.
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