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公开(公告)号：EP1278767A1
公开(公告)日：2003-01-29
申请号：EP01934875.4
申请日：2001-04-12
发明人： BALLANCE, David, James , SLEEP, Darrell , TURNER, Andrew, John , SADEGHI, Homayoun , PRIOR, Christopher, P.
CPC分类号： A61K38/4846 , A01K2217/05 , A61K9/0019 , A61K38/21 , A61K38/212 , A61K38/38 , A61K47/42 , A61K47/65 , A61K48/00 , A61K2039/54 , C07K14/56 , C07K14/61 , C07K14/62 , C07K14/65 , C07K14/705 , C07K14/7151 , C07K14/76 , C07K14/765 , C07K2319/00 , C07K2319/21 , C07K2319/31 , C07K2319/50 , C07K2319/75 , C12N9/96 , C12N15/62 , Y02A50/386 , Y02A50/401 , Y02A50/412
摘要： The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention and using these nucleic acids, vectors, and/or host cells. Additionally the present invention encompasses pharmaceutical compositions comprising albumin fusion proteins and methods of treating, preventing, or ameliorating diseases, disorders or conditions using albumin fusion proteins of the invention.
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公开(公告)号：EP1149163A2
公开(公告)日：2001-10-31
申请号：EP00901252.7
申请日：2000-01-31
发明人： VAN URK, Hendrik,Delta Biotechnology Limited , MEAD, David John,Delta Biotechnology Limited , MORTON, Philip Harvey,Delta Biotechnology Limited , CARTWRIGHT, Andrew John,Delta Biotechnology Ltd , CAMERON, Jason,Delta Biotechnology Limited , BALLANCE, David James,Aventis Behring , GRANDGEORGE, Michel G. J.,Aventis Behring GmbH , BEREZENKO, Stephen,Delta Biotechnology Limited , WOODROW, John Rodney,Delta Biotechnology Limited , SLEEP, Darrell,Delta Biotechnology Limited , VERON, Jean-Luc Bernard,Delta Biotechnology Ltd
CPC分类号： B01D15/3804 , B01D15/362 , B01D15/363 , C07K14/76 , C07K14/765
摘要： A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.
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公开(公告)号：EP0764209B1
公开(公告)日：2001-01-10
申请号：EP95921063.4
申请日：1995-06-07
发明人： WOOD, Patricia Carol, Delta Biotechnology Limited , QUIRK, Alan Victor, Delta Biotechnology Limited
CPC分类号： C07K14/765
摘要： Reduction (preferably elimination) of the HSP150 protein in a yeast used to produce desired foreign proteins, especially human albumin, facilitates subsequent purification of the protein.
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公开(公告)号：EP0870039A1
公开(公告)日：1998-10-14
申请号：EP96942515.0
申请日：1996-12-19
CPC分类号： A61K38/27 , C07K14/61 , C07K14/765 , C07K2319/00
摘要： Fusion proteins of albumin and growth hormone, or fusions of variants of either, are secreted well in yeast and have increased serum and storage stability.
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5.发明授权
公开(公告)号：EP0399666B1
公开(公告)日：1993-07-28
申请号：EP90304575.5
申请日：1990-04-26
CPC分类号： C07K14/435 , A61K38/00 , C07K14/78 , C07K2319/20 , C07K2319/31 , C12N15/62
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公开(公告)号：EP0258067B1
公开(公告)日：1993-03-24
申请号：EP87307668.1
申请日：1987-08-28
CPC分类号： C12N15/81 , C07K14/765
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公开(公告)号：EP0407008A3
公开(公告)日：1991-07-17
申请号：EP90304574.8
申请日：1990-04-26
发明人： Geisow, Michael John
CPC分类号： C07K14/435 , A61K38/00 , C07K14/78 , C07K2319/20 , C07K2319/31 , C12N15/62
摘要： The 140 kD fragment or a variant thereof generated by the action of the enzyme cathepsin D on human plasma fibronectin is used therapeutically for wound healing, especially on the cornea.
摘要翻译： 通过组织蛋白酶D对人血浆纤连蛋白的作用产生的140kD片段或其变体用于治疗伤口愈合，尤其是在角膜上。
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公开(公告)号：EP0431880A1
公开(公告)日：1991-06-12
申请号：EP90313129.0
申请日：1990-12-04
CPC分类号： C07K14/745 , C07K14/8125 , C07K14/8132
摘要： Mutant transformed fungal (eg yeast) strains producing high levels of heterologous protein may be detected by plating fungal cells onto a solid medium such as agarose and applying antibodies to the heterologous protein. The resulting complexes can either be visualised directly or following staining or labelling.
摘要翻译： 可以通过将真菌细胞铺在固体培养基如琼脂糖上并将抗体应用于异源蛋白质来检测产生高水平异源蛋白质的突变转化真菌（例如酵母）菌株。 所得到的复合物可以直接显示或染色或标记。
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公开(公告)号：EP0424117A1
公开(公告)日：1991-04-24
申请号：EP90311381.9
申请日：1990-10-17
CPC分类号： C12N9/2428 , C07K14/765 , C12N15/81
摘要： A yeast promoter, believed to be that for glycerol-3-phosphate dehydrogenase, has been sequenced and characterised and can be used to regulate expression of heterologous genes in yeast. In particular, expression can thereby be depressed by addition of glycerol or ethanol to the fermentation medium.
摘要翻译： 被认为是甘油-3-磷酸脱氢酶的酵母启动子已被测序和表征，可用于调节酵母中异源基因的表达。 特别地，通过向发酵培养基中加入甘油或乙醇可以抑制表达。
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公开(公告)号：EP0258067A3
公开(公告)日：1989-05-10
申请号：EP87307668.1
申请日：1987-08-28
CPC分类号： C12N15/81 , C07K14/765
摘要： A hybrid yeast promoter comprising constituents of the 5ʹ non-coding PGK region and, as an upstream activation sequence, the upstream activation sequence of the GAL 10 gene of Saccharomyces cerevisiae , and which does not contain the endogenous PGK upstream activation sequence. Preferably the GAL 10 upstream activation sequence is provided at a site from which the PGK upstream activation sequence has been deleted. The hybrid promoter confers galactose regulation of gene transcription.
摘要翻译： 杂交酵母启动子包含5'非编码PGK区域的组成部分，和作为上游激活序列的酿酒酵母GAL10基因的上游激活序列，并且其不含内源PGK上游激活序列。 优选地，GAL10上游激活序列在PGK上游激活序列已经从其中缺失的位点提供。 杂合启动子赋予半乳糖调节基因转录。
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