发明公开
EP0397097A1 Cultivation of transformed microorganisms
失效
Züchtungvon transformierten Mikroorganismen。
- 专利标题： Cultivation of transformed microorganisms
- 专利标题（中）： Züchtungvon transformierten Mikroorganismen。
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申请号： EP90108609.0申请日： 1990-05-08
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公开(公告)号： EP0397097A1公开(公告)日： 1990-11-14
- 发明人: Terasawa, Masato , Yamagata, Hisashi , Yukawa, Hideaki , Fukushima, Makiko , Kurusu, Yasurou
- 申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL
- 申请人地址： 16-9, 2-chome, Shiba Minato-ku Tokyo JP
- 专利权人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL
- 当前专利权人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL
- 当前专利权人地址： 16-9, 2-chome, Shiba Minato-ku Tokyo JP
- 代理机构： Kraus, Walter, Dr.
- 优先权： JP113847/89 19890508; JP45724/90 19900228; JP47192/90 19900301
- 主分类号： C12N15/60
- IPC分类号： C12N15/60 ; C12P13/22 ; C12N9/88 ; C12N1/21
摘要：
A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can express by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3 %, and thereby allowing the desired structural gene to express in the microorganism.
公开/授权文献
- EP0397097B1 Cultivation of transformed microorganisms 公开/授权日：1995-02-08
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