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    Cultivation of transformed microorganisms
    3.
    发明公开
    Cultivation of transformed microorganisms 失效
    Züchtungvon transformierten Mikroorganismen。

    公开(公告)号：EP0397097A1

    公开(公告)日：1990-11-14

    申请号：EP90108609.0

    申请日：1990-05-08

    申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL

    发明人： Terasawa, Masato ,  Yamagata, Hisashi ,  Yukawa, Hideaki ,  Fukushima, Makiko ,  Kurusu, Yasurou

    IPC分类号： C12N15/60 ,  C12P13/22 ,  C12N9/88 ,  C12N1/21

    CPC分类号： C12N9/88 ,  C12P13/227 ,  Y10S435/849

    摘要： A process for cultivating a microorganism transformed with a recombinant plasmid at least contain­ing (a) a DNA fragment containing a promoter and a regu­lator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment con­taining a desired structural gene which can express by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3 %, and thereby allowing the desired structural gene to express in the microorganism.

    摘要翻译： 培养用重组质粒转化的微生物的方法，所述重组质粒至少含有（a）含有启动子的DNA片段和位于启动子下游的色氨酸酶操纵子（tna）中的调节基因tnaC的方法，和（b）含有 可以由启动子表达的所需结构基因，其包括在连续或间歇地添加作为碳源的葡萄糖的同时在培养基中培养转化的微生物，使得葡萄糖的浓度保持在0.01〜0.3％的范围内，从而允许 在微生物中表达的所需结构基因。

    Process for producing L-Tryptophan
    5.
    发明公开
    Process for producing L-Tryptophan 失效
    Verfahren zur Herstellung von L-色氨酸。

    公开(公告)号：EP0295622A2

    公开(公告)日：1988-12-21

    申请号：EP88109445.2

    申请日：1988-06-14

    申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL

    发明人： Yamagata, Hisashi ,  Endo, Fuzio ,  Shimazu, Mitsunobu ,  Terasawa, Masato ,  Yukawa, Hideaki

    IPC分类号： C12P13/22 ,  C12N9/88

    CPC分类号： C12P13/227 ,  C12N9/88

    摘要： A process for producing L-tryptophan optionally substituted at the 5-position by a hydroxyl group, which comprises reacting fumaric acid or its salt, an ammonium ion and indole optionally substituted at the 5-position by a hydroxyl group in the presence of (A) fumarase, (B) pyruvic acid-malic acid carboxylase, (C) tryptophanase and (D) NADH oxidase.

    摘要翻译： 一种通过羟基任选在5位被取代的L-色氨酸的方法，该方法包括在（A）存在下使富马酸或其盐，任选地在5位被羟基取代的铵离子和吲哚 ）富马酸酶，（B）丙酮酸 - 苹果酸羧化酶，（C）色氨酸酶和（D）NADH氧化酶。