发明公开
EP0502589A2 Kit for use in amplifying and detecting nucleic acid sequences
失效
Kit zur Verwendung bei der Amplifizierung und beim Nachweis vonNukleinsäuresequenzen。
- 专利标题： Kit for use in amplifying and detecting nucleic acid sequences
- 专利标题（中）： Kit zur Verwendung bei der Amplifizierung und beim Nachweis vonNukleinsäuresequenzen。
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申请号： EP92201245.5申请日： 1986-03-27
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公开(公告)号： EP0502589A2公开(公告)日： 1992-09-09
- 发明人: Mullis, Kary Banks , Arnheim, Norman , Saiki, Randall Keichi , Erlich, Henry Anthony , Horn, Glenn Thomas , Scharf, Stephen Joel
- 申请人： F. HOFFMANN-LA ROCHE AG
- 申请人地址： Postfach 3255 CH-4002 Basel CH
- 专利权人： F. HOFFMANN-LA ROCHE AG
- 当前专利权人： F. HOFFMANN-LA ROCHE AG
- 当前专利权人地址： Postfach 3255 CH-4002 Basel CH
- 代理机构： Jaenichen, Hans-Rainer, Dr.
- 优先权： US716975 19850328; US791308 19851025; US828144 19860207
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
The present invention is directed to a kit for the amplification and detection of at least one specific nucleic acid sequence in a sample, which kit comprises in packaged form, a multicontainer unit comprising:
(a) primer for each different specific nucleic acid sequence being amplified and detected, selected so as to provide a primer substantially complementary to each strand of each specific sequence such that the extension product synthesized from one primer, when it is separated from its complement, serves as a template for synthesis of an extension product of the other primer;
(b) means for synthesizing primer extension products; and
(c) means for detecting the amplified sequence or sequences.
(a) primer for each different specific nucleic acid sequence being amplified and detected, selected so as to provide a primer substantially complementary to each strand of each specific sequence such that the extension product synthesized from one primer, when it is separated from its complement, serves as a template for synthesis of an extension product of the other primer;
(b) means for synthesizing primer extension products; and
(c) means for detecting the amplified sequence or sequences.
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