发明公开
EP0561082A1 Methods of regeneration of medicago sativa and expressing foreign DNA in same
失效
Verfahren zur Regeneration von Medicago sativa und Expression fremder DNS in diesem Medicago sativa。
- 专利标题： Methods of regeneration of medicago sativa and expressing foreign DNA in same
- 专利标题（中）： Verfahren zur Regeneration von Medicago sativa und Expression fremder DNS in diesem Medicago sativa。
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申请号： EP92311126.4申请日： 1992-12-07
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公开(公告)号： EP0561082A1公开(公告)日： 1993-09-22
- 发明人: Buising, Charisse Marie , Tomes, Thomas , Schmidt, Janice
- 申请人： PIONEER HI-BRED INTERNATIONAL, INC.
- 申请人地址： 700 Capital Square 400 Locust Street Des Moines Iowa 50309 US
- 专利权人： PIONEER HI-BRED INTERNATIONAL, INC.
- 当前专利权人： PIONEER HI-BRED INTERNATIONAL, INC.
- 当前专利权人地址： 700 Capital Square 400 Locust Street Des Moines Iowa 50309 US
- 代理机构： Goldin, Douglas Michael
- 优先权： US817205 19920106
- 主分类号： C12N15/87
- IPC分类号： C12N15/87 ; C12N15/82 ; C12N5/14 ; C12N5/04 ; A01H1/00 ; A01H4/00
摘要：
The invention relates to improved transformation and regeneration of alfalfa, Medicago sativa .
A method is used to transform alfalfa by using particle acceleration. Optimum results with mature cotyledons occurs when bombarding after 24 to 120 hours of imbibing water. Regeneration and transformation of alfalfa is greatly improved by using immature cotyledons or embryos of immature cotyledons for transformation and regeneration Immature cotyledons include those up to about 25 days past pollination, and preferably include cotyledons excised at 10-15 days past pollination, most preferably including those excised at about 10 days past pollination. These cotyledons have a light green to translucent appearance. Plants resulting from bombardment of somatic embryos of immature cotyledons retain regeneration ability.
A method is used to transform alfalfa by using particle acceleration. Optimum results with mature cotyledons occurs when bombarding after 24 to 120 hours of imbibing water. Regeneration and transformation of alfalfa is greatly improved by using immature cotyledons or embryos of immature cotyledons for transformation and regeneration Immature cotyledons include those up to about 25 days past pollination, and preferably include cotyledons excised at 10-15 days past pollination, most preferably including those excised at about 10 days past pollination. These cotyledons have a light green to translucent appearance. Plants resulting from bombardment of somatic embryos of immature cotyledons retain regeneration ability.
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