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    Tissue culture method for transformation of plant cells
    1.
    发明公开
    Tissue culture method for transformation of plant cells 失效
    Pflanzengewebekulturverfahren zur转型von Pflanzenzellen。

    公开(公告)号：EP0424047A1

    公开(公告)日：1991-04-24

    申请号：EP90311229.0

    申请日：1990-10-12

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Tomes, Dwight ,  Bidney, Dennis ,  Buising, Charisse Marie

    IPC分类号： C12N15/87 ,  C12N15/82

    CPC分类号： C12N15/8207 ,  C12N15/8201 ,  C12N15/87

    摘要： Plant cells in tissue culture are more efficiently transformed in a method which involves treatment with a cytokinin followed by incubation for a period sufficient to permit undifferentiated cells in cotyledonary node tissue to differentiate into meristematic cells and to permit the cells to enter the phases between the G1 and division phases of development.

    摘要翻译： 组织培养中的植物细胞在一种方法中被更有效地转化，该方法包括用细胞分裂素处理，然后孵育足以允许子叶节点组织中的未分化细胞分化为分生细胞并允许细胞进入G1期 和开发阶段。

    Methods of regeneration of medicago sativa and expressing foreign DNA in same
    4.
    发明公开
    Methods of regeneration of medicago sativa and expressing foreign DNA in same 失效
    Verfahren zur Regeneration von Medicago sativa und Expression fremder DNS in diesem Medicago sativa。

    公开(公告)号：EP0561082A1

    公开(公告)日：1993-09-22

    申请号：EP92311126.4

    申请日：1992-12-07

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Buising, Charisse Marie ,  Tomes, Thomas ,  Schmidt, Janice

    IPC分类号： C12N15/87 ,  C12N15/82 ,  C12N5/14 ,  C12N5/04 ,  A01H1/00 ,  A01H4/00

    CPC分类号： A01H4/008 ,  A01H1/00 ,  A01H4/00 ,  C12N15/8207

    摘要： The invention relates to improved transformation and regeneration of alfalfa, Medicago sativa .
    A method is used to transform alfalfa by using particle acceleration. Optimum results with mature cotyledons occurs when bombarding after 24 to 120 hours of imbibing water. Regeneration and transformation of alfalfa is greatly improved by using immature cotyledons or embryos of immature cotyledons for transformation and regeneration Immature cotyledons include those up to about 25 days past pollination, and preferably include cotyledons excised at 10-15 days past pollination, most preferably including those excised at about 10 days past pollination. These cotyledons have a light green to translucent appearance. Plants resulting from bombardment of somatic embryos of immature cotyledons retain regeneration ability.

    摘要翻译： 本发明涉及改进苜蓿，苜蓿的转化和再生。 一种使用粒子加速度来转换苜蓿的方法。 成熟子叶的最佳结果发生在24至120小时吸水后进行轰击。 通过使用未成熟的子叶或未成熟子叶的胚胎进行转化和再生，可以大大提高苜蓿的再生和转化。未成熟子叶包括从授粉到大约25天的子叶，优选包括在授粉后10-15天切除的子叶，最优选包括那些 在授粉约10天后切除。 这些子叶具有浅绿色至半透明的外观。 由未成熟子叶的体细胞胚发生的植物保留再生能力。