发明公开
- 专利标题: METHOD AND KITS FOR PREPARING MULTICOMPONENT NUCLEIC ACID CONSTRUCTS
- 专利标题(中): 方法和试剂盒制备多组分核酸构建
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申请号: EP97930083.0申请日: 1997-06-16
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公开(公告)号: EP0915903A1公开(公告)日: 1999-05-19
- 发明人: HARNEY, Peter, D.
- 申请人: Biodynamics Associates
- 申请人地址: 56 Damien Road Wellesley Hills, MA 02181 US
- 专利权人: Biodynamics Associates
- 当前专利权人: Biodynamics Associates
- 当前专利权人地址: 56 Damien Road Wellesley Hills, MA 02181 US
- 代理机构: Horner, Martin Grenville, et al
- 优先权: US19960019869P 19960617
- 国际公布: WO1997048716 19971224
- 主分类号: C12N15
- IPC分类号: C12N15 ; C07H21
摘要:
The invention provides a highly efficient, rapid, and cost effective method of linking nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct. Such a method and multicomponent construct are illustrated in the figure. The invention further provides nucleic acid components, each nucleic acid component comprising a double stranded nucleic acid molecule having at least one single stranded 5' or 3' terminal sequence, the terminal sequence having sufficient complementarity to either a terminal sequence in a separate nucleic acid component or a sequence in a linking nucleic acid molecule so as to allow for specific annealing of complementary sequences and linkage of the components in a predetermined order. Kits containing reagents required to practice the method of the invention are also provided.
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