发明公开
EP0915903A1 METHOD AND KITS FOR PREPARING MULTICOMPONENT NUCLEIC ACID CONSTRUCTS 失效
方法和试剂盒制备多组分核酸构建

  • 专利标题: METHOD AND KITS FOR PREPARING MULTICOMPONENT NUCLEIC ACID CONSTRUCTS
  • 专利标题(中): 方法和试剂盒制备多组分核酸构建
  • 申请号: EP97930083.0
    申请日: 1997-06-16
  • 公开(公告)号: EP0915903A1
    公开(公告)日: 1999-05-19
  • 发明人: HARNEY, Peter, D.
  • 申请人: Biodynamics Associates
  • 申请人地址: 56 Damien Road Wellesley Hills, MA 02181 US
  • 专利权人: Biodynamics Associates
  • 当前专利权人: Biodynamics Associates
  • 当前专利权人地址: 56 Damien Road Wellesley Hills, MA 02181 US
  • 代理机构: Horner, Martin Grenville, et al
  • 优先权: US19960019869P 19960617
  • 国际公布: WO1997048716 19971224
  • 主分类号: C12N15
  • IPC分类号: C12N15 C07H21
METHOD AND KITS FOR PREPARING MULTICOMPONENT NUCLEIC ACID CONSTRUCTS
摘要:
The invention provides a highly efficient, rapid, and cost effective method of linking nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct. Such a method and multicomponent construct are illustrated in the figure. The invention further provides nucleic acid components, each nucleic acid component comprising a double stranded nucleic acid molecule having at least one single stranded 5' or 3' terminal sequence, the terminal sequence having sufficient complementarity to either a terminal sequence in a separate nucleic acid component or a sequence in a linking nucleic acid molecule so as to allow for specific annealing of complementary sequences and linkage of the components in a predetermined order. Kits containing reagents required to practice the method of the invention are also provided.
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