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公开(公告)号：EP0915903A4
公开(公告)日：2002-09-04
申请号：EP97930083
申请日：1997-06-16
发明人： HARNEY PETER D
CPC分类号： C12N15/64 , C07H21/00 , C12N15/1093 , C12N15/66
摘要： The invention provides a highly efficient, rapid, and cost effective method of linking nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct. Such a method and multicomponent construct are illustrated in the figure. The invention further provides nucleic acid components, each nucleic acid component comprising a double stranded nucleic acid molecule having at least one single stranded 5' or 3' terminal sequence, the terminal sequence having sufficient complementarity to either a terminal sequence in a separate nucleic acid component or a sequence in a linking nucleic acid molecule so as to allow for specific annealing of complementary sequences and linkage of the components in a predetermined order. Kits containing reagents required to practice the method of the invention are also provided.
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公开(公告)号：EP0915903A1
公开(公告)日：1999-05-19
申请号：EP97930083.0
申请日：1997-06-16
发明人： HARNEY, Peter, D.
CPC分类号： C12N15/64 , C07H21/00 , C12N15/1093 , C12N15/66
摘要： The invention provides a highly efficient, rapid, and cost effective method of linking nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct. Such a method and multicomponent construct are illustrated in the figure. The invention further provides nucleic acid components, each nucleic acid component comprising a double stranded nucleic acid molecule having at least one single stranded 5' or 3' terminal sequence, the terminal sequence having sufficient complementarity to either a terminal sequence in a separate nucleic acid component or a sequence in a linking nucleic acid molecule so as to allow for specific annealing of complementary sequences and linkage of the components in a predetermined order. Kits containing reagents required to practice the method of the invention are also provided.
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