发明授权
EP1025194B1 BLEACHING COMPOSITIONS COMPRISING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS
有权
漂白剂组合物含有多种取代的蛋白酶变
- 专利标题: BLEACHING COMPOSITIONS COMPRISING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS
- 专利标题(中): 漂白剂组合物含有多种取代的蛋白酶变
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申请号: EP98955079.3申请日: 1998-10-23
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公开(公告)号: EP1025194B1公开(公告)日: 2008-12-10
- 发明人: GHOSH, Chanchal, Kumar , BAECK, Andre, Cesar , OHTANI, Ryohei , BUSCH, Alfred , SHOWELL, Michael, Stanford , SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , POULOSE, Ayrookaran, J.
- 申请人: GENENCOR INTERNATIONAL, INC.
- 申请人地址: 925 Page Mill Road Palo Alto, California 94304-1013 US
- 专利权人: GENENCOR INTERNATIONAL, INC.
- 当前专利权人: GENENCOR INTERNATIONAL, INC.
- 当前专利权人地址: 925 Page Mill Road Palo Alto, California 94304-1013 US
- 代理机构: Forrest, Graham Robert
- 优先权: US956323 19971023; US956564 19971023; US956324 19971023
- 国际公布: WO1999020726 19990429
- 主分类号: C12N9/54
- IPC分类号: C12N9/54
摘要:
The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.
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