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    BLEACHING COMPOSITIONS COMPRISING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS
    3.
    发明授权
    BLEACHING COMPOSITIONS COMPRISING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 有权
    漂白剂组合物含有多种取代的蛋白酶变

    公开(公告)号：EP1025194B2

    公开(公告)日：2012-01-04

    申请号：EP98955079.3

    申请日：1998-10-23

    申请人： GENENCOR INTERNATIONAL, INC.

    发明人： GHOSH, Chanchal, Kumar ,  BAECK, Andre, Cesar ,  OHTANI, Ryohei ,  BUSCH, Alfred ,  SHOWELL, Michael, Stanford ,  SCHELLENBERGER, Volker ,  KELLIS, James, T., Jr. ,  PAECH, Christian ,  NADHERNY, Joanne ,  NAKI, Donald, P. ,  COLLIER, Katherine, D. ,  CALDWELL, Robert, M. ,  POULOSE, Ayrookaran, J.

    IPC分类号： C12N9/54

    CPC分类号： C12N9/54 ,  A23G4/123 ,  A23K20/189 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/3907 ,  C11D3/3945 ,  C12Y304/00 ,  C12Y304/21062

    摘要： The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.

    MASS SPECTROMETRIC ANALYSIS OF BIOPOLYMERS
    4.
    发明授权
    MASS SPECTROMETRIC ANALYSIS OF BIOPOLYMERS 有权
    生物聚合物的质量分析

    公开(公告)号：EP1311707B1

    公开(公告)日：2008-10-15

    申请号：EP01964178.6

    申请日：2001-08-17

    申请人： GENENCOR INTERNATIONAL, INC.

    发明人： PAECH, Christian ,  PAECH, Sigrid ,  ESTELL, David, A. ,  GANSHAW, Grant, C.

    IPC分类号： C12Q1/68

    CPC分类号： H01J49/0036 ,  Y10T436/24 ,  Y10T436/25125

    摘要： The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separatioln technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.

    MULTIPLY-SUBSTITUTED PROTEASE VARIANTS
    6.
    发明授权
    MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 有权
    多取代蛋白酶变

    公开(公告)号：EP1025241B2

    公开(公告)日：2009-10-21

    申请号：EP98956181.6

    申请日：1998-10-23

    申请人： GENENCOR INTERNATIONAL, INC. ,  THE PROCTER & GAMBLE COMPANY

    发明人： SCHELLENBERGER, Volker ,  KELLIS, James, T., Jr. ,  PAECH, Christian ,  NADHERNY, Joanne ,  NAKI, Donald, P. ,  POULOSE, Ayrookaran, J. ,  COLLIER, Katherine, D. ,  CALDWELL, Robert, M. ,  BAECK, André, C.

    IPC分类号： C12N15/57 ,  C12N9/54 ,  C11D3/386 ,  A23K1/165

    CPC分类号： C12N9/54 ,  A23G4/123 ,  A23K20/189 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/3907 ,  C11D3/3945 ,  C12Y304/00 ,  C12Y304/21062

    摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin.

    MASS SPECTROMETRIC ANALYSIS OF BIOPOLYMERS
    7.
    发明公开
    MASS SPECTROMETRIC ANALYSIS OF BIOPOLYMERS 有权
    生物聚合物的质量分析

    公开(公告)号：EP1311707A2

    公开(公告)日：2003-05-21

    申请号：EP01964178.6

    申请日：2001-08-17

    申请人： Genencor International, Inc.

    发明人： PAECH, Christian ,  PAECH, Sigrid ,  ESTELL, David, A. ,  GANSHAW, Grant, C.

    IPC分类号： C12Q1/68

    CPC分类号： H01J49/0036 ,  Y10T436/24 ,  Y10T436/25125

    摘要： The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separatioln technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.

    MULTIPLY-SUBSTITUTED PROTEASE VARIANTS
    8.
    发明公开
    MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 有权
    多取代蛋白酶变

    公开(公告)号：EP1025241A2

    公开(公告)日：2000-08-09

    申请号：EP98956181.6

    申请日：1998-10-23

    申请人： GENENCOR INTERNATIONAL INC. ,  THE PROCTER & GAMBLE COMPANY

    发明人： SCHELLENBERGER, Volker ,  KELLIS, James, T., Jr. ,  PAECH, Christian ,  NADHERNY, Joanne ,  NAKI, Donald, P. ,  POULOSE, Ayrookaran, J. ,  COLLIER, Katherine, D. ,  CALDWELL, Robert, M. ,  BAECK, André, C.

    IPC分类号： C12N15/57 ,  C12N9/54 ,  C11D3/386 ,  A23K1/165

    CPC分类号： C12N9/54 ,  A23G4/123 ,  A23K20/189 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/3907 ,  C11D3/3945 ,  C12Y304/00 ,  C12Y304/21062

    摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin.