发明公开
EP1133573A2 GENOTYPING BY MASS SPECTROMETRIC ANALYSIS OF SHORT DNA FRAGMENTS
审中-公开
基因芯片技术用于短链DNA片段的质谱分析
- 专利标题： GENOTYPING BY MASS SPECTROMETRIC ANALYSIS OF SHORT DNA FRAGMENTS
- 专利标题（中）： 基因芯片技术用于短链DNA片段的质谱分析
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申请号： EP99960522.3申请日： 1999-11-22
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公开(公告)号： EP1133573A2公开(公告)日： 2001-09-19
- 发明人: LAKEN, Steven, J. , VOGELSTEIN, Bert , KINZLER, Kenneth, W. , GROOPMAN, John, D. , JACKSON, Peta, E. , FRIESEN, Marlin, D.
- 申请人： The Johns Hopkins University
- 申请人地址： Suite 906,111 Market Place Baltimore, MD 21202 US
- 专利权人： The Johns Hopkins University
- 当前专利权人： The Johns Hopkins University
- 当前专利权人地址： Suite 906,111 Market Place Baltimore, MD 21202 US
- 代理机构： Tombling, Adrian George
- 优先权： US198340 19981124
- 国际公布： WO0031300 20000602
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N30/72 ; G01N33/50
摘要：
Genotyping can be accomplished by analysis of short, defined DNA segments using electrospray ionization mass spectrometry. The DNA segments are produced using specially designed primers to amplify a cDNA or genomic DNA template. The primers contain a recognition site for a restriction endonuclease. The amplification products are digested with the restriction endonuclease. Single nucleotide polymorphisms can be detected rapidly and reliably.
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