公开(公告)号：EP1133573A2

公开(公告)日：2001-09-19

申请号：EP99960522.3

申请日：1999-11-22

申请人： The Johns Hopkins University

发明人： LAKEN, Steven, J. ,  VOGELSTEIN, Bert ,  KINZLER, Kenneth, W. ,  GROOPMAN, John, D. ,  JACKSON, Peta, E. ,  FRIESEN, Marlin, D.

IPC分类号： C12Q1/68 ,  G01N30/72 ,  G01N33/50

CPC分类号： C12Q1/683 ,  C12Q1/6844 ,  C12Q1/6872 ,  C12Q2565/627 ,  C12Q2525/204 ,  C12Q2521/313 ,  C12Q2525/131

摘要： Genotyping can be accomplished by analysis of short, defined DNA segments using electrospray ionization mass spectrometry. The DNA segments are produced using specially designed primers to amplify a cDNA or genomic DNA template. The primers contain a recognition site for a restriction endonuclease. The amplification products are digested with the restriction endonuclease. Single nucleotide polymorphisms can be detected rapidly and reliably.

摘要翻译： 基因分型可以通过使用电喷雾电离质谱法分析短的，确定的DNA片段来完成。 使用特别设计的引物来产生DNA片段以扩增cDNA或基因组DNA模板。 引物含有限制性内切核酸酶的识别位点。 扩增产物用限制性内切酶消化。 单核苷酸多态性可以快速可靠地检测到。

公开(公告)号：EP1075660A1

公开(公告)日：2001-02-14

申请号：EP99922892.7

申请日：1999-05-10

申请人： The Johns Hopkins University

发明人： ANDERSON, Charles, W. ,  BARGERON, C., Brent ,  BENSON, Richard, C. ,  CARLSON, Micah, A. ,  FRASER, Alan, B. ,  GROOPMAN, John, D. ,  KO, Harvey, W. ,  KOHLER, David, R. ,  PHILLIPS, Terry, E. ,  STRICKLAND, Paul, T.

IPC分类号： G01N33/536

CPC分类号： G01N1/40 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2400/0481 ,  G01N1/405 ,  G01N21/6428 ,  G01N21/645 ,  G01N30/02 ,  G01N33/54373 ,  G01N33/582 ,  G01N2001/022 ,  G01N2201/0221 ,  B01D15/3804

摘要： A hand-held, self-contained, automatic, low power and rapid sensor platform for detecting and quantifying a plurality of analytes. A sample solution potentially containing an unknown amount of an analyte is passed through an affinity column which contains antibodies to which the analyte binds thereby extracting the analyte. The affinity column is then rinsed to remove any other chemicals that may fluoresce. The rinsed affinity column is then eluted with a known volume of elution fluid causing the analyte to release from the antibody and dissolve in the fluid (eluant). The eluant is then placed in the quartz cuvette of a fluorometer. The analyte suspended in the eluant fluoresces at a waveband which is different than that of the light source that excites it. The amount of fluorescence is measured and the level of analyte determined. The rinsing and elution fluids, and/or the affinity column can be placed in a module for easy insertion and removal from the sensor when depleted or when the sensor is to be used against a different analyte.

摘要翻译： 一种手持式，自包含式，自动化，低功耗和快速的传感器平台，用于检测和量化多种分析物。 可能含有未知量分析物的样品溶液通过亲和柱，该柱含有分析物结合的抗体，从而提取分析物。 然后漂洗亲和柱以除去可能发荧光的任何其他化学物质。 然后用已知体积的洗脱液洗脱漂洗过的亲和柱，使分析物从抗体中释放出来并溶解在流体中（洗脱液）。 然后将洗脱液置于荧光计的石英比色杯中。 悬浮在洗脱液中的分析物在不同于激发它的光源的波段处发荧光。 测量荧光量并确定分析物的水平。 清洗和洗脱液和/或亲和柱可放置在模块中，以便在耗尽时或当传感器用于抵抗不同分析物时易于从传感器插入和移除。