发明授权
- 专利标题: PHAGE-DEPENDENT SUPER-PRODUCTION OF BIOLOGICALLY ACTIVE PROTEINS AND PEPTIDES
- 专利标题(中): 生物活性肽和蛋白质噬菌体依赖超级量产
-
申请号: EP00932819.6申请日: 2000-05-24
-
公开(公告)号: EP1180153B1公开(公告)日: 2005-04-13
- 发明人: KORDYUM, Vitaliy, A. , CHERNYKH, Svetlana, I. , SLAVCHENKO, Irina, Y. , VOZIANOV, Oleksandr, F.
- 申请人: Phage Biotechnology Corporation
- 申请人地址: 18300 Von Karman Avenue, Suite 440 Irvine, CA 92612 US
- 专利权人: Phage Biotechnology Corporation
- 当前专利权人: Phage Biotechnology Corporation
- 当前专利权人地址: 18300 Von Karman Avenue, Suite 440 Irvine, CA 92612 US
- 代理机构: De Clercq, Ann
- 优先权: US318288 19990525
- 国际公布: WO2000071731 20001130
- 主分类号: C12N15/73
- IPC分类号: C12N15/73 ; C07K14/60 ; C12P21/02 ; C12N7/00 ; C12N1/06
摘要:
This invention relates to a method for enhancing the production of soluble and biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage μ with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.
公开/授权文献
信息查询
IPC分类: