-
1.发明公开
公开(公告)号：EP1180153A1
公开(公告)日：2002-02-20
申请号：EP00932819.6
申请日：2000-05-24
发明人： KORDYUM, Vitaliy, A. , CHERNYKH, Svetlana, I. , SLAVCHENKO, Irina, Y. , VOZIANOV, Oleksandr, F.
摘要： This invention relates to a method for enhancing the production of soluble and biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage μ with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.
-
2.发明授权
公开(公告)号：EP1180153B1
公开(公告)日：2005-04-13
申请号：EP00932819.6
申请日：2000-05-24
发明人： KORDYUM, Vitaliy, A. , CHERNYKH, Svetlana, I. , SLAVCHENKO, Irina, Y. , VOZIANOV, Oleksandr, F.
摘要： This invention relates to a method for enhancing the production of soluble and biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage μ with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.
-
搜索结果
2 条记录 (耗时 0.008 秒)
