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141 条记录 (耗时 0.039 秒)

    ANTIBIOTIC-FREE PLASMID
    2.
    发明公开
    ANTIBIOTIC-FREE PLASMID 有权
    抗生素无质粒

    公开(公告)号：EP2432884A1

    公开(公告)日：2012-03-28

    申请号：EP10720510.6

    申请日：2010-05-24

    申请人： Merial Ltd.

    发明人： AUDONNET, Jean-christophe ,  JOLIVET, Edmond

    IPC分类号： C12N15/73 ,  C12N15/63 ,  C12N15/68

    CPC分类号： C12N15/73 ,  C12N15/635 ,  C12N15/85 ,  C12N2800/107 ,  C12N2800/24 ,  C12P21/00

    摘要： The present invention provides a method of maintaining a gram negative bacterium plasmid without the use of antibiotic selection pressure. Further, the invention relates to the drugless plasmids produced including drugless plasmids containing a heterologous gene. The invention also provides formulations and/or compositions comprising the drugless plasmids comprising a heterologous gene, formulations and/or compositions comprising a protein or an immunogen expressed using the drugless plasmids, and methods of administering such formulations and/or compositions to a host. The invention relates to gram negative bacteria containing the drugless plasmids.

    GENERATION OF RECOMBINANT GENES IN BACTERIOPHAGES
    8.
    发明公开
    GENERATION OF RECOMBINANT GENES IN BACTERIOPHAGES 审中-公开
    产生重组基因在噬菌体

    公开(公告)号：EP1763579A1

    公开(公告)日：2007-03-21

    申请号：EP05758007.8

    申请日：2005-07-06

    申请人： Mixis France S.A.

    发明人： LUQUE, Alejandro ,  STROBEL, Heike ,  MARTINSOHN, Jann, Thorsten ,  PETIT, Marie-Agnès ,  RADMAN, Miroslav

    IPC分类号： C12N15/10 ,  C12N15/73

    CPC分类号： C12N15/73

    摘要： The present invention relates to in vivo methods for generating and detecting recombinant DNA sequences in bacteriophages or plasmids containing bacteriophage sequences, methods for generating hybrid genes and hybrid proteins encoded by these hybrid genes by the use of bacteriophages and plasmids containing bacteriophage sequences, bacteriophages and plasmids that can be used in these methods, and kits comprising appropriate bacterial host cells and bacteriophages or plasmids. DNA sequences for which these methods are relevant include protein-encoding and non-coding sequences.

    A METHOD OF PRODUCING BIOLOGICALLY ACTIVE HUMAN ACIDIC FIBROBLAST GROWTH FACTOR AND ITS USE IN PROMOTING ANGIOGENESIS
    9.
    发明公开
    A METHOD OF PRODUCING BIOLOGICALLY ACTIVE HUMAN ACIDIC FIBROBLAST GROWTH FACTOR AND ITS USE IN PROMOTING ANGIOGENESIS 审中-公开
    PROCESS FOR用于促进血管生成PRODUCING生物活性的人酸性成纤维细胞生长因子及其用途

    公开(公告)号：EP1309716A4

    公开(公告)日：2005-08-03

    申请号：EP01967977

    申请日：2001-08-15

    申请人： PHAGE BIOTECHNOLOGY CORP

    发明人： STEGMANN THOMAS J ,  KORDYUM VITALIY A ,  CHERNYKH SVITLANA I ,  SLAVCHENKO IRYNA YU ,  VOZIANOV OLEKSANDR F

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P9/00 ,  C07K14/50 ,  C12N1/21 ,  C12N7/01 ,  C12N15/70 ,  C12N15/73 ,  C12P21/02 ,  C12P21/00 ,  C07H21/04 ,  C12N7/00

    CPC分类号： C07K14/501 ,  C12N15/70

    摘要： The gene of human acidic fibroblast growth factor 155 (haFGF 155) has been obtained by chemical synthesis. The nucleotide sequence of haFGF 155 gene has been deduced on the basis of haFGF 155 amino acid sequence as described in the literature. The amino acid sequence, of the synthesized haFGF 155 does not differ from those described in the literature. The nucleotide sequence of haFGF gene differs from those described previously. For chemical synthesis of haFGF 155 gene, codons were used which are the ones most often used by E. coli in highly expressed E. coli proteins. A plasmid with haFGF 155 (phaFGF 155) gene was obtained and was used to transform E. coli. Production of haFGF 154 protein was achieved by cultivation of the producer strain under conditions which slow down the lytic development of lambda phage. The haFGF 154 protein accumulated in culture medium in a soluble condition as a result of the producer strain cells lysis by the lambda phage. The haFGF 154 protein constituted 20% of the soluble protein accumulated in the culture medium and its biological activity was demonstrated by its ability to generate new vessels (angiogenesis). The initiator methionine residue at position 1 of the FGF 155 protein was completely removed during protein synthesis resulting in an FGF 154 amino acid product. The use of the phage-dependent method to produce other forms of the haFGF protein is also disclosed.

    CLONING VECTORS AND METHOD FOR MOLECULAR CLONING
    10.
    发明公开
    CLONING VECTORS AND METHOD FOR MOLECULAR CLONING 审中-公开
    分子克隆和方法克隆

    公开(公告)号：EP1366177A1

    公开(公告)日：2003-12-03

    申请号：EP02712474.2

    申请日：2002-02-25

    申请人： Riken

    发明人： HAYASHIZAKI, Yoshihide ,  CARNINCI, Piero

    IPC分类号： C12N15/73

    CPC分类号： C12N15/73 ,  C12N15/65

    摘要： The invention discloses a family of cloning vectors capable of cloning nucleic acid inserts of interest of long sizes, with low or reduced background and high efficiency of excision and method for preparing these vectors and library thereof.As example, it is disclosed a cloning vector comprising a construction vector segment (CS) and a replaceable segment (RS), wherein the size of CS is: 36.5 kb ≤ CS ≤ 38 kb, preferably CS is 37.5 kb, comprising lox recombination sites for Cre-recombination and/or att recombination sites for Gateway-like recombination, preferably also a background-reducing system selected from the group of: the ccdB gene, a lox sequence, the lacZ gene, and asymmetric site sequences recognized by restriction endonucleases.