发明授权
EP1365643B1 MICROINJECTION ASSEMBLY AND METHODS FOR MICROINJECTING AND REIMPLANTING AVIAN EGGS
有权
“微注射装置和方法微注塑和韵律人工林鸟蛋”
- 专利标题： MICROINJECTION ASSEMBLY AND METHODS FOR MICROINJECTING AND REIMPLANTING AVIAN EGGS
- 专利标题（中）： “微注射装置和方法微注塑和韵律人工林鸟蛋”
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申请号： EP02718905.9申请日： 2002-02-06
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公开(公告)号： EP1365643B1公开(公告)日： 2008-08-06
- 发明人: CHRISTMANN, Leandro
- 申请人： Avigenics, Inc.
- 申请人地址： 111 Riverbend Road Athens, GA 30605 US
- 专利权人： Avigenics, Inc.
- 当前专利权人： Avigenics, Inc.
- 当前专利权人地址： 111 Riverbend Road Athens, GA 30605 US
- 代理机构： Silveston, Judith
- 优先权： US269012P 20010213; US919143 20010731
- 国际公布： WO2002064727 20020822
- 主分类号： C12N15/89
- IPC分类号： C12N15/89 ; C12M3/00 ; A01K45/00 ; A01K67/027
摘要：
The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.
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