公开(公告)号：EP1365643B1

公开(公告)日：2008-08-06

申请号：EP02718905.9

申请日：2002-02-06

申请人： Avigenics, Inc.

发明人： CHRISTMANN, Leandro

IPC分类号： C12N15/89 ,  C12M3/00 ,  A01K45/00 ,  A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12M35/00 ,  G02B21/32

摘要： The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.

公开(公告)号：EP1364205B1

公开(公告)日：2007-05-23

申请号：EP02713487.3

申请日：2002-01-28

申请人： Avigenics, Inc.

发明人： RAPP, Jeffrey, C.

IPC分类号： G01N33/00 ,  A01K67/027 ,  C12N5/00 ,  C12N5/02 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85

CPC分类号： C12N15/8509 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/02 ,  C12N15/873 ,  C12N2799/022 ,  C12N2799/027 ,  C12N2840/203

摘要： The present invention relates generally to novel methods of producing transgenic chickens that generate antibodies or immunoglobulin polypeptides in whites of eggs. More specifically, one embodiment of the present invention relates to methods of inserting immunoglobulin-encoding transgenes into avian sperm cells for transfer to ova to generate transgenic zygotes. The transgenes may include at least two immunoglobulin-encoding nucleic acid sequences and an internal ribosome entry site (IRES) that allow the immunoglobulin polypeptides to be expressed by chicken cells and hence in egg whites.

公开(公告)号：EP1708564A4

公开(公告)日：2007-03-07

申请号：EP04749444

申请日：2004-03-26

申请人： AVIGENICS INC

发明人： RAPP JEFFREY ,  CHRISTMANN LEANDRO ,  HARVEY ALEX ,  LEAVITT MARKLEY

IPC分类号： A01K67/00 ,  A01K67/027 ,  C12N15/00 ,  C12N15/85 ,  C12N15/90

CPC分类号： C12N15/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/02 ,  C12N15/8509

摘要： The invention provides methods for integrating a heterologous polynucleotide into the genome of an avian cell. The methods deliver to an avian cell a polynucleotide and a source of integrase activity that mediates recombination between the polynucleotide and the genomic DNA of the avian cell. The invention provides modified avian or artificial chromosomes as vectors to shuttle transgenes or gene clusters into an avian genome. Another aspect of the invention are avian cells genetically modified with a transgene vector. One cell line for the delivery and integration of a transgene comprises a heterologous attP site and, optionally, a region for expressing the integrase. Methods are also included for the production of a heterologous polypeptide by transgenic avian tissue involve integrating a heterologous polynucleotide into the avian genome. The present invention also relates to methods of producing transgenic chickens which include introducing into an avian cell a nucleic acid comprising a transgene and an integrase activity in addition to a cationic polymer and/or a nuclear localization signal and introducing the avian cell into a recipient avian wherein the recipient avian produces an offspring which includes the transgene. Also included are methods of dispersing a nucleic acid in a cell.

公开(公告)号：EP1937294A1

公开(公告)日：2008-07-02

申请号：EP06826502.4

申请日：2006-10-23

申请人： Avigenics, Inc.

发明人： YESLAND, Kyle, D.

IPC分类号： A61K38/17 ,  A61K38/21

CPC分类号： A61K38/212 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/01 ,  A61K38/1816 ,  A61K38/193 ,  A61K38/215 ,  A61K47/60 ,  C12N15/8509

摘要： Compositions containing a glycosylated therapeutic amino acid sequence obtained from a transgenic avian wherein the therapeutic amino acid sequence is a glycoprotein and includes a covalently bonded glycol polymer.

公开(公告)号：EP1931698A1

公开(公告)日：2008-06-18

申请号：EP06815963.1

申请日：2006-10-03

申请人： Avigenics, Inc.

发明人： HARVEY, Alex J.

IPC分类号： C07K14/16 ,  C07K19/00

CPC分类号： C12N15/86 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/01 ,  C07K14/505 ,  C07K14/56 ,  C12N15/8509 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2799/027

摘要： The invention includes methods of producing viral particles which include introducing into avian cells a nucleotide sequence encoding a replication deficient retroviral vector and introducing into the avian cells nucleotide sequences encoding products required for replication of the replication deficient retroviral vector under the control of a promoter that is functional in the avian cell line, and harvesting the viral particles.

公开(公告)号：EP1708564A2

公开(公告)日：2006-10-11

申请号：EP04749444.8

申请日：2004-03-26

申请人： Avigenics, Inc.

发明人： RAPP, Jeffrey ,  CHRISTMANN, Leandro ,  HARVEY, Alex ,  LEAVITT, Markley

IPC分类号： A01K67/00 ,  C12N15/00

CPC分类号： C12N15/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/02 ,  C12N15/8509

摘要： The invention provides methods for integrating a heterologous polynucleotide into the genome of an avian cell. The methods deliver to an avian cell a polynucleotide and a source of integrase activity that mediates recombination between the polynucleotide and the genomic DNA of the avian cell. The invention provides modified avian or artificial chromosomes as vectors to shuttle transgenes or gene clusters into an avian genome. Another aspect of the invention are avian cells genetically modified with a transgene vector. One cell line for the delivery and integration of a transgene comprises a heterologous attP site and, optionally, a region for expressing the integrase. Methods are also included for the production of a heterologous polypeptide by transgenic avian tissue involve integrating a heterologous polynucleotide into the avian genome. The present invention also relates to methods of producing transgenic chickens which include introducing into an avian cell a nucleic acid comprising a transgene and an integrase activity in addition to a cationic polymer and/or a nuclear localization signal and introducing the avian cell into a recipient avian wherein the recipient avian produces an offspring which includes the transgene. Also included are methods of dispersing a nucleic acid in a cell.

公开(公告)号：EP1633781A2

公开(公告)日：2006-03-15

申请号：EP04817319.9

申请日：2004-05-28

申请人： Avigenics, Inc.

发明人： HARVEY, Alex J., AviGenics, Inc. ,  LEAVITT, Markley C., AviGenics, Inc. ,  WANG, Youliang, AviGenics, Inc.

IPC分类号： C07K14/56

CPC分类号： C07K14/56 ,  A01K67/0275 ,  A01K2217/206 ,  A01K2227/30 ,  C07K14/81 ,  C07K2317/732 ,  C07K2317/734 ,  C12N15/8509 ,  C12N2800/204

摘要： The present invention includes nucleic acid molecules comprising an artificial chromosome and an avian ovomucoid gene expression controlling region operably linked to the coding sequence of a useful polypeptide.

公开(公告)号：EP1461430A4

公开(公告)日：2005-04-13

申请号：EP02786838

申请日：2002-12-02

申请人： AVIGENICS INC

发明人： HARVEY ALEX J ,  WANG YOULIANG

IPC分类号： A01K67/027 ,  A01K67/00 ,  C07K14/56 ,  C07K14/81 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N15/09 ,  C12N15/11 ,  C12N15/85

CPC分类号： C07K14/8135 ,  A01K2227/30 ,  C07K14/56 ,  C12N2799/021

公开(公告)号：EP1503627A2

公开(公告)日：2005-02-09

申请号：EP03724449.8

申请日：2003-05-02

申请人： Avigenics, Inc.

发明人： HARVEY, Alex, J.

IPC分类号： A21C11/16

CPC分类号： C12N15/1003 ,  C12Q1/6806 ,  C12Q2537/149 ,  C12Q2527/125

摘要： Genetic modification or selection of avians requires that large numbers of birds be genetically analyzed for sequences of interest. Typically, DNA is extracted on an individual basis from samples taken from the birds. Current methods of DNA extraction extract the DNA from blood or other tissue using tedious and time-consuming procedures. The present invention provides a high throughput screening assay for detecting a genetic sequence in multiple samples. The assay further provides a DNA extraction method that allows DNA to be extracted rapidly from multiple avian samples, such as red blood cells. The extraction method is extremely reliable and does not require that each sample be quantitated post-extraction. The extracted DNA can be used for a variety of genetic assays, including a high throughput screening assay to identify insertions of a transgene. The present invention is particularly useful for extracting DNA from nucleated RBCs. Therefore, the method can be applied towards genetic analysis of avians, fish, reptiles and amphibians.

公开(公告)号：EP1478751A2

公开(公告)日：2004-11-24

申请号：EP02725432.5

申请日：2002-03-29

申请人： Avigenics, Inc.

发明人： RAPP, Jeffrey, C.

IPC分类号： C12N15/11

CPC分类号： C12N15/85 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/01 ,  C07K14/56 ,  C07K16/2818 ,  C07K2317/20 ,  C07K2317/21 ,  C07K2317/23 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/46 ,  C12N2830/90

摘要： The present invention provides novel isolated nucleic acids comprising an avian nucleic acid sequence encoding a lysozyme gene expression control region. The isolated nucleic acid of the present invention is useful for reducing the chromosomal positional effect of a transgene operably linked to the lysozyme gene expression control region and transfected into a recipient cell and allows expression of an operably linked heterologous nucleic acid insert in a transfected avian cells such as, for example, an oviduct cell. The isolated avian lysozyme of the present invention may be operably linked with a selected nucleic acid insert encoding a polypeptide desired to be expressed in a transfected cell. The recombinant DNA of the present invention may further comprise a polyadenylation signal sequence or a chicken lysozyme 3' domain.