公开(公告)号：EP1365643B1

公开(公告)日：2008-08-06

申请号：EP02718905.9

申请日：2002-02-06

申请人： Avigenics, Inc.

发明人： CHRISTMANN, Leandro

IPC分类号： C12N15/89 ,  C12M3/00 ,  A01K45/00 ,  A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12M35/00 ,  G02B21/32

摘要： The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.

公开(公告)号：EP1708564A2

公开(公告)日：2006-10-11

申请号：EP04749444.8

申请日：2004-03-26

申请人： Avigenics, Inc.

发明人： RAPP, Jeffrey ,  CHRISTMANN, Leandro ,  HARVEY, Alex ,  LEAVITT, Markley

IPC分类号： A01K67/00 ,  C12N15/00

CPC分类号： C12N15/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/02 ,  C12N15/8509

摘要： The invention provides methods for integrating a heterologous polynucleotide into the genome of an avian cell. The methods deliver to an avian cell a polynucleotide and a source of integrase activity that mediates recombination between the polynucleotide and the genomic DNA of the avian cell. The invention provides modified avian or artificial chromosomes as vectors to shuttle transgenes or gene clusters into an avian genome. Another aspect of the invention are avian cells genetically modified with a transgene vector. One cell line for the delivery and integration of a transgene comprises a heterologous attP site and, optionally, a region for expressing the integrase. Methods are also included for the production of a heterologous polypeptide by transgenic avian tissue involve integrating a heterologous polynucleotide into the avian genome. The present invention also relates to methods of producing transgenic chickens which include introducing into an avian cell a nucleic acid comprising a transgene and an integrase activity in addition to a cationic polymer and/or a nuclear localization signal and introducing the avian cell into a recipient avian wherein the recipient avian produces an offspring which includes the transgene. Also included are methods of dispersing a nucleic acid in a cell.

公开(公告)号：EP1313849B1

公开(公告)日：2006-06-07

申请号：EP01968166.7

申请日：2001-08-27

申请人： Avigenics, Inc.

发明人： CHRISTMANN, Leandro ,  PRATT, Scott, L. ,  RAPP, Jeffrey, C.

IPC分类号： C12N15/00 ,  A01K67/027

CPC分类号： C12N15/8509 ,  A01K67/0273 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/30 ,  A01K2267/01 ,  A01K2267/02 ,  C07K14/005 ,  C12N2770/24222

摘要： Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.

公开(公告)号：EP1608219A2

公开(公告)日：2005-12-28

申请号：EP04716170.8

申请日：2004-03-01

申请人： Avigenics, Inc.

发明人： HARVEY, Alex ,  LEAVITT, Markley, C. ,  CHRISTMANN, Leandro

IPC分类号： A01K67/00

CPC分类号： A01K67/0275 ,  A01K2217/072 ,  A01K2227/30 ,  A01K2267/01 ,  C12N15/8509 ,  C12N2800/204 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2830/40 ,  C12N2830/90 ,  C12N2840/20 ,  C12N2840/203

摘要： The invention provides methods for integrating a heterologous polynucleotide into the genome of an avian cell. The methods deliver to an avian cell a polynucleotide and a source of integrase activity that mediates recombination between the polynucleotides and the genomic DNA of the avian cell. The invention provides modified avian or artificial chromosomes as vectors to shuttle transgenes or gene clusters into an avian genome. Another aspect of the invention are avian cells genetically modified with a transgene vector. One cell line for the delivery and integration of a transgene comprises a heterologous attP site and, optionally, a region for expressing the integrase. Methods are also included for the production of a heterologous polypeptide by transgenic avian tissue involve integrating a heterologous polynucleotide into the avian genome. Subsequently, a mature transgenic avian is derived by transferring the transgenic blastodermal cells to an embryo and allowing that embryo to develop fully.

公开(公告)号：EP1313849B8

公开(公告)日：2007-03-07

申请号：EP01968166.7

申请日：2001-08-27

申请人： Avigenics, Inc.

发明人： CHRISTMANN, Leandro ,  PRATT, Scott, L. ,  RAPP, Jeffrey, C.

IPC分类号： C12N15/00 ,  A01K67/027

CPC分类号： C12N15/8509 ,  A01K67/0273 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/30 ,  A01K2267/01 ,  A01K2267/02 ,  C07K14/005 ,  C12N2770/24222

摘要： Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.

公开(公告)号：EP1725091A1

公开(公告)日：2006-11-29

申请号：EP05723783.6

申请日：2005-02-28

申请人： Avigenics, Inc.

发明人： CHRISTMANN, Leandro ,  EBERHARDT, Dawn ,  HARVEY, Alex, J. ,  LEAVITT, Markley, C.

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/07 ,  A01K2227/30 ,  A01K2267/00 ,  A01K2267/01 ,  C12N15/8509 ,  C12N15/907 ,  C12N2800/90 ,  C12N2840/203

摘要： The invention includes transchromosomal avians and transchromosomal avian cells and methods for the introduction of artificial chromosomes into the genome of avians and avian cells.

公开(公告)号：EP1438401A2

公开(公告)日：2004-07-21

申请号：EP02775895.2

申请日：2002-09-18

申请人： Avigenics, Inc.

发明人： RAPP, Jeffrey, C. ,  CHRISTMANN, Leandro

IPC分类号： C12N15/00

CPC分类号： A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/20 ,  C07K2317/23 ,  C12N15/8509 ,  C12N2799/027 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/46 ,  C12N2830/90 ,  C12N2840/203

摘要： This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.

公开(公告)号：EP1365643A2

公开(公告)日：2003-12-03

申请号：EP02718905.9

申请日：2002-02-06

申请人： Avigenics, Inc.

发明人： CHRISTMANN, Leandro

IPC分类号： A01K45/00 ,  A61K48/00 ,  C12N15/00

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12M35/00 ,  G02B21/32

摘要： The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.

公开(公告)号：EP1313849A2

公开(公告)日：2003-05-28

申请号：EP01968166.7

申请日：2001-08-27

申请人： Avigenics, Inc.

发明人： CHRISTMANN, Leandro ,  PRATT, Scott, L. ,  RAPP, Jeffrey, C.

IPC分类号： C12N15/00 ,  A01K67/027

CPC分类号： C12N15/8509 ,  A01K67/0273 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/30 ,  A01K2267/01 ,  A01K2267/02 ,  C07K14/005 ,  C12N2770/24222

摘要： Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.