The present invention relates a promoter capable of strongly expressing a gene. linked downstream (3' side) of the promoter in prokaryote, especially in Escherichia Coli . The promoter of the invention is a promoter for prokaryote having a novel nucleotide sequence between any -35 region sequence and any -10 region sequence. The promoter has a strong promoter activity and thus a target peptide or protein can be produced at a high efficiency and in large amounts through linking the structural gene encoding the target peptide or protein downstream of the promoter.