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公开(公告)号：EP1391518A1
公开(公告)日：2004-02-25
申请号：EP02769430.6
申请日：2002-05-16
摘要： It is an objective of the present invention to provide a novel gene recombination-based method for producing desired peptides that allows effective, large scale production of the desired peptides.
According to the method of the present invention, excision of peptides of interest is achieved by the use of the right-handed scissors ( i.e. , S-cyanylation reaction) along with the left-handed scissors ( i.e. , treatment with cyanogen bromide, enterokinase, factor Xa, or the like). The method utilizes the excision technique in conjunction with the tandem repeat technique and thereby provides a useful gene recombination-based technique for peptide synthesis that is particularly effective in the large-scale synthesis of low-molecular weight peptides.摘要翻译： 本发明的目的是提供一种新的基于基因重组的方法，用于产生所需肽，其允许有效，大规模生产所需的肽。 根据本发明的方法，通过使用右旋剪刀（即，S-氰化反应）以及左手剪刀（即，用氰酸处理）来实现目的肽的切除 溴化物，肠激酶，因子Xa等）。 该方法利用与串联重复技术相结合的切除技术，从而为肽合成提供了有用的基于基因重组的技术，其在低分子量肽的大规模合成中特别有效。
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公开(公告)号：EP1304376A1
公开(公告)日：2003-04-23
申请号：EP01984291.3
申请日：2001-07-25
发明人： ITO, Takashi , TANAKA, Yoko , KONDO, Mitsuyo
CPC分类号： C12P21/02
摘要： It is intended to produce a target protein in procaryotic or eucaryotic cells. A DNA encoding the full-length amino acid sequence or a part of the same of a target protein is prepared and the target protein is produced by a gene recombination technique with the use of this DNA.
摘要翻译： 它旨在在原核或真核细胞中产生靶蛋白。 制备编码靶蛋白全长氨基酸序列或其一部分的DNA，利用该DNA通过基因重组技术产生靶蛋白。
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公开(公告)号：EP1148129A1
公开(公告)日：2001-10-24
申请号：EP99959698.4
申请日：1999-12-08
发明人： ITO, Takashi , KONDO, Mitsuyo , TANAKA, Yoko , KOBAYASHI, Masayuki , IGARASHI, Koichi , SASADA, Reiko , NISHIMURA, Osamu
CPC分类号： C07K14/475 , A61K38/00 , C07K14/485
摘要： The betacellulin mutein or its salt of the present invention is useful for better therapeutic drug for diabetes, since they have intact BTC activity and reduced EGF activity, and no antigenicity-related problems.
摘要翻译： 本发明的细胞素突变蛋白或其盐可用于更好的糖尿病治疗药物，因为它们具有完整的BTC活性和降低的EGF活性，并且没有抗原性相关的问题。
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公开(公告)号：EP1142908A1
公开(公告)日：2001-10-10
申请号：EP00900127.2
申请日：2000-01-06
发明人： ITO, Takashi , TANAKA, Yoko , KAISHO, Tomoko , NISHIMURA, Osamu
CPC分类号： C07K14/523 , A61K38/00
摘要： The present invention relates to a process for activating a purified polypeptide, characterized by bringing the purified polypeptide having an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 1 into contact with copper ions, calcium ions or magnesium ions, or a substance providing copper ions, a substance providing calcium ions, or a substance providing magnesium ions.
The methods for activation or manufacturing of the present invention enable the inexpensive mass production of active MPIF-1Δ23 which protects myeloid stem cells from chemotherapeutic drugs and permits rapid recovery from leukopenia, which is a limiting factor in the administration of anti-tumor agents.摘要翻译： 本发明涉及一种活化纯化多肽的方法，其特征在于将具有与SEQ ID NO：1所示的氨基酸序列相同或基本相同的氨基酸序列的纯化多肽与铜离子，钙离子或镁接触 离子或提供铜离子的物质，提供钙离子的物质，或提供镁离子的物质。 本发明的激活或制造方法使得能够廉价地批量生产活性MPIF-1 DELTA 23，其保护骨髓干细胞免受化学治疗药物的作用，并允许从白细胞减少症中快速恢复，这是施用抗肿瘤剂的限制因素 。
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公开(公告)号：EP1138772A1
公开(公告)日：2001-10-04
申请号：EP99961284.9
申请日：1999-12-09
发明人： SUENAGA, Masato , ITO, Takashi , NISHIMURA, Osamu
IPC分类号： C12N15/62 , C12N15/12 , C12N15/18 , C12P21/02 , C12N1/21 , C07K19/00 , C07K14/47 , C07K14/50 , C07K1/107 , C07K1/12
CPC分类号： C07K14/47 , C07K2319/00
摘要： The present invention provides a production method advantageous in producing Apelin on an industrial scale in large amounts.
The present invention relates to a method for producing Apelin or a salt thereof, comprising subjecting a fused protein or peptide consisting of a protein or peptide having N-terminal cysteine with Apelin optionally having an optionally oxidized methionine residue at the N-terminus thereof joined to the N-terminus of the protein or peptide to a cleavage reaction for the peptide bond on the amino group side of the cysteine residue.
Using the production method of the present invention, it is possible to produce on an industrial scale in large amounts Apelin that can be used as a therapeutic/preventive agent for such diseases as various forms of dementia, including senile dementia and cerebrovascular dementia, depression, hyperkinetic (minimal brain damage) syndrome, disturbance of consciousness, anxiety disorder, schizophrenia, phobia, impairments of growth hormone secretion, hyperphagia, polyphagia, hypercholesterolemia, hyperglyceridemia, hyperlipidemia, hyperprolactinemia, hypoglycemia, hypopituitarism, pituitary dwarfism, diabetes mellitus, cancers, pancreatitis, renal diseases, Turner's syndrome, neurosis, rheumatoid arthritis, spinal injury, transient cerebral ischemic attack, amyotrophic lateral sclerosis, acute myocardial infarction, spinocerebellar deformation, bone fractures, wounds, atopic dermatitis, osteoporosis, asthma, epilepsy, infertility, arteriosclerosis, pulmonary emphysema, pulmonary edema, and milk secretion insufficiency, and can also be used as a hypnotic sedative, a postoperative nutritional status improving agent, a hypertensive, a hypotensive, a preventive or therapeutic drug for HIV infection, AIDS, etc., and the like.摘要翻译： 本发明提供了在工业规模上大量生产Apelin的生产方法。 本发明涉及一种生产Apelin或其盐的方法，其包括将具有N-末端半胱氨酸的蛋白质或肽组成的融合蛋白或肽与任选具有任选氧化的甲硫氨酸残基的Apelin在其N末端连接至 蛋白质或肽的N-末端为半胱氨酸残基的氨基侧上的肽键的裂解反应。 使用本发明的制造方法，可以以工业规模生产大量的可以用作各种形式的老年痴呆症，诸如老年性痴呆，脑血管性痴呆，抑郁症等疾病的治疗预防剂的Apelin， 超动力学（最小脑损伤）综合征，意识障碍，焦虑障碍，精神分裂症，恐惧症，生长激素分泌的损伤，食欲不振，多食，高胆固醇血症，高甘油血症，高脂血症，高催乳素血症，低血糖症，垂体功能减退症，垂体侏儒症，糖尿病， ，肾脏疾病，特纳综合征，神经症，类风湿关节炎，脊髓损伤，短暂性脑缺血发作，肌萎缩性侧索硬化，急性心肌梗死，脊髓小脑变形，骨折，创伤，特应性皮炎，骨质疏松症，哮喘，癫痫，不孕，动脉硬化，肺 肺气肿，肺水肿， d乳分泌不足，也可用作催眠镇静剂，术后营养状态改善剂，高血压药，降血糖药，艾滋病毒感染预防或治疗药物，艾滋病等。
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公开(公告)号：EP1400593A1
公开(公告)日：2004-03-24
申请号：EP02733320.2
申请日：2002-06-05
发明人： ITO, Takashi , TANAKA, Yoko , KURIYAMA, Masato
CPC分类号： C12N15/67 , C07K14/245 , C12N15/70 , C12N15/71 , C12P21/02
摘要： The present invention relates a promoter capable of strongly expressing a gene. linked downstream (3' side) of the promoter in prokaryote, especially in Escherichia Coli . The promoter of the invention is a promoter for prokaryote having a novel nucleotide sequence between any -35 region sequence and any -10 region sequence. The promoter has a strong promoter activity and thus a target peptide or protein can be produced at a high efficiency and in large amounts through linking the structural gene encoding the target peptide or protein downstream of the promoter.
摘要翻译： 本发明涉及能够强烈表达基因的启动子。 在原核生物中，尤其是大肠杆菌中的启动子的下游（3'侧）连接。 本发明的启动子是原核生物的启动子，其具有任何-35个区域序列和任何-10个区域序列之间的新的核苷酸序列。 启动子具有很强的启动子活性，因此通过将编码启动子下游的靶肽或蛋白质的结构基因连接起来，可以以高效率和大量产生靶肽或蛋白质。
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