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    NOVEL PROMOTER
    1.
    发明公开
    NOVEL PROMOTER 审中-公开
    中性推荐机构

    公开(公告)号：EP1400593A1

    公开(公告)日：2004-03-24

    申请号：EP02733320.2

    申请日：2002-06-05

    申请人： Takeda Chemical Industries, Ltd.

    发明人： ITO, Takashi ,  TANAKA, Yoko ,  KURIYAMA, Masato

    IPC分类号： C12N15/67 ,  C12N1/21 ,  C12P21/00

    CPC分类号： C12N15/67 ,  C07K14/245 ,  C12N15/70 ,  C12N15/71 ,  C12P21/02

    摘要： The present invention relates a promoter capable of strongly expressing a gene. linked downstream (3' side) of the promoter in prokaryote, especially in Escherichia Coli . The promoter of the invention is a promoter for prokaryote having a novel nucleotide sequence between any -35 region sequence and any -10 region sequence. The promoter has a strong promoter activity and thus a target peptide or protein can be produced at a high efficiency and in large amounts through linking the structural gene encoding the target peptide or protein downstream of the promoter.

    摘要翻译： 本发明涉及能够强烈表达基因的启动子。 在原核生物中，尤其是大肠杆菌中的启动子的下游（3'侧）连接。 本发明的启动子是原核生物的启动子，其具有任何-35个区域序列和任何-10个区域序列之间的新的核苷酸序列。 启动子具有很强的启动子活性，因此通过将编码启动子下游的靶肽或蛋白质的结构基因连接起来，可以以高效率和大量产生靶肽或蛋白质。

    PROCESS FOR PRODUCING RECOMBINANT PROTEIN
    2.
    发明公开
    PROCESS FOR PRODUCING RECOMBINANT PROTEIN 审中-公开
    生产重组蛋白的方法

    公开(公告)号：EP1304376A1

    公开(公告)日：2003-04-23

    申请号：EP01984291.3

    申请日：2001-07-25

    申请人： Takeda Chemical Industries, Ltd.

    发明人： ITO, Takashi ,  TANAKA, Yoko ,  KONDO, Mitsuyo

    IPC分类号： C12N15/10 ,  C12N1/21 ,  C12P21/02 ,  C12Q1/02

    CPC分类号： C12P21/02

    摘要： It is intended to produce a target protein in procaryotic or eucaryotic cells. A DNA encoding the full-length amino acid sequence or a part of the same of a target protein is prepared and the target protein is produced by a gene recombination technique with the use of this DNA.

    摘要翻译： 它旨在在原核或真核细胞中产生靶蛋白。 制备编码靶蛋白全长氨基酸序列或其一部分的DNA，利用该DNA通过基因重组技术产生靶蛋白。

    PROCESS FOR PRODUCING ACTIVATED PROTEIN
    4.
    发明公开
    PROCESS FOR PRODUCING ACTIVATED PROTEIN 审中-公开
    方法ZUM HERSTELLEN VON AKTIVIERTEN PROTEINEN

    公开(公告)号：EP1142908A1

    公开(公告)日：2001-10-10

    申请号：EP00900127.2

    申请日：2000-01-06

    申请人： Takeda Chemical Industries, Ltd.

    发明人： ITO, Takashi ,  TANAKA, Yoko ,  KAISHO, Tomoko ,  NISHIMURA, Osamu

    IPC分类号： C07K14/47 ,  C12P21/02 ,  C12N15/12

    CPC分类号： C07K14/523 ,  A61K38/00

    摘要： The present invention relates to a process for activating a purified polypeptide, characterized by bringing the purified polypeptide having an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID NO: 1 into contact with copper ions, calcium ions or magnesium ions, or a substance providing copper ions, a substance providing calcium ions, or a substance providing magnesium ions.
    The methods for activation or manufacturing of the present invention enable the inexpensive mass production of active MPIF-1Δ23 which protects myeloid stem cells from chemotherapeutic drugs and permits rapid recovery from leukopenia, which is a limiting factor in the administration of anti-tumor agents.

    摘要翻译： 本发明涉及一种活化纯化多肽的方法，其特征在于将具有与SEQ ID NO：1所示的氨基酸序列相同或基本相同的氨基酸序列的纯化多肽与铜离子，钙离子或镁接触 离子或提供铜离子的物质，提供钙离子的物质，或提供镁离子的物质。 本发明的激活或制造方法使得能够廉价地批量生产活性MPIF-1 DELTA 23，其保护骨髓干细胞免受化学治疗药物的作用，并允许从白细胞减少症中快速恢复，这是施用抗肿瘤剂的限制因素 。