发明授权
EP1423528B1 VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSGRADES VON BESTIMMTEN CYTOSINEN IN GENOMISCHER DNA IM SEQUENZKONTEXT 5'-CPG-3'
有权
PROCEDURE决定某些甲基化的胞嘧啶到基因组DNA序列的程度在上下文中5'-CPG-3'
- 专利标题： VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSGRADES VON BESTIMMTEN CYTOSINEN IN GENOMISCHER DNA IM SEQUENZKONTEXT 5'-CPG-3'
- 专利标题（英）： Method for determining the degree of methylation of defined cytosines in genomic dna in the sequence context 5'-cpg-3'
- 专利标题（中）： PROCEDURE决定某些甲基化的胞嘧啶到基因组DNA序列的程度在上下文中5'-CPG-3'
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申请号： EP01980315.4申请日： 2001-09-01
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公开(公告)号： EP1423528B1公开(公告)日： 2008-01-02
- 发明人: OLEK, Alexander , PIEPENBROCK, Christian , BERLIN, Kurt , GÜTIG, David
- 申请人： Epigenomics AG
- 申请人地址： Kleine Präsidentenstrasse 1 10178 Berlin DE
- 专利权人： Epigenomics AG
- 当前专利权人： Epigenomics AG
- 当前专利权人地址： Kleine Präsidentenstrasse 1 10178 Berlin DE
- 代理机构： Schubert, Klemens
- 优先权： DE10043826 20000901; DE10044543 20000905
- 国际公布： WO2002018632 20020307
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The invention relates to a method for detecting the degree of methylation of a defined cytosine in the sequence context 5′-CpG-3′ of a genomic DNA sample. The first stage involves chemically treating the genomic DNA in such a way that the cytosine bases, but not the 5-methylcytosine bases, are converted into uracil. Parts of the genomic DNA containing the defined cytosine are then amplified. The amplified parts are given a detectable mark and the extent of the hybridisation of the amplified parts on the two classes of oligonucleotides is then determined by detecting the mark of the amplified parts. The degree of methylation of the defined cytosine in the genomic DNA sample can be deduced on the basis of the relationship between the marks detected on the two classes of oligonucleotides following the hybridisation.
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