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    A DISTRIBUTED SYSTEM FOR EPIGENETIC BASED PREDICTION OF COMPLEX PHENOTYPES
    2.
    发明公开
    A DISTRIBUTED SYSTEM FOR EPIGENETIC BASED PREDICTION OF COMPLEX PHENOTYPES 审中-公开
    分布式SYSTEM FOR络合物表型的表观遗传学-BASED预测

    公开(公告)号：EP1502222A2

    公开(公告)日：2005-02-02

    申请号：EP02743199.8

    申请日：2002-06-14

    申请人： Epigenomics AG

    发明人： ADORJAN, Peter ,  OLEK, Alexander ,  PIEPENBROCK, Christian

    IPC分类号： G06F19/00

    CPC分类号： G06F19/28 ,  G06F19/18 ,  G06F19/24

    摘要： This invention concerns systems for collecting and storing epigenetic and phenotypic information about samples in order to measure and analyse tissue samples and/or cell lines, where the epigenetic parameter is DNA methylation and the phenotypic parameters describe an individual. The method includes parameters such as the diagnosis of diseases and/or drug resistance, wherein the correlation of the epigenetic with the phenotypic parameters is done substantially without human intervention.

    VERFAHREN ZUR QUANTIFIZIERUNG VON CYTOSIN-METHYLIERUNGEN IN KOMPLEX AMPLIFIZIERTER GENOMISCHER DNA
    3.
    发明公开
    VERFAHREN ZUR QUANTIFIZIERUNG VON CYTOSIN-METHYLIERUNGEN IN KOMPLEX AMPLIFIZIERTER GENOMISCHER DNA 审中-公开
    定量胞嘧啶甲基化方法在复杂基因组DNA扩增

    公开(公告)号：EP1366190A2

    公开(公告)日：2003-12-03

    申请号：EP01989384.1

    申请日：2001-12-05

    申请人： Epigenomics AG

    发明人： OLEK, Alexander

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/683 ,  C12Q1/6874 ,  C12Q2565/631

    摘要： The invention relates to a method for providing demethylated DNA as a reference material for analyzing cytosine methylations in genomic DNA samples while using complex amplifications. To this end, the following method steps are carried out: a) a genomic DNA sample is amplified with primers that are either very short or degenerated oligonucleotides or oligonucleotides that are complementary to adapters. In the second case, the DNA sample is cut by a restriction enzyme before amplification, and the adapters, understood as short nucleotide fragments of a known sequence, ligate to the ends of the resulting DNA fragments. The amplificates are chemically treated in such a manner that cytosine bases, which are unmethylated at the 5-position, are transformed into uracil, thymine or into another base that is dissimilar to cytosine with regard to hybridization behavior, whereas the 5-methylcytosine bases remain essentially unchanged. This is understood in the following as chemical pretreatment. The chemically pretreated amplificates are, in turn, amplified. To this end, a number of specifically hybridizing oligonucleotides or oligonucleotides that are complementary to the adapters are used as primers. In the latter case, the chemical pretreatment is also carried out.

    VERFAHREN ZUM NACHWEIS VON CYTOSINMETHYLIERUNGSMUSTERN MIT HOHER SENSITIVITÄT
    7.
    发明公开
    VERFAHREN ZUM NACHWEIS VON CYTOSINMETHYLIERUNGSMUSTERN MIT HOHER SENSITIVITÄT 有权
    方法检测灵敏度CYTOSINMETHYLIERUNGSMUSTERN高

    公开(公告)号：EP1370691A2

    公开(公告)日：2003-12-17

    申请号：EP02722194.4

    申请日：2002-03-08

    申请人： Epigenomics AG

    发明人： OLEK, Alexander ,  BERLIN, Kurt

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2523/125 ,  C12Q2537/163

    摘要： The invention relates to a method for detecting cytosine methylation in DNA samples. The inventive method comprises the following steps: a genomic DNA sample containing DNA to be examined and background DNA is chemically treated in such a way that all non-methylated cytosine bases are converted into uracil, the 5-methylcytosine bases remaining unchanged; the chemically treated DNA samples are amplified using at least two primer oligonucleotides and one polymerase, the DNA to be examined being preferred as a template as opposed to the background DNA; the amplificates are analysed; and the methylation status in the DNA to be examined is inferred from the presence of an amplificate and/or from the analysis of further positions.