发明公开
EP1458240A2 NUCLEIC ACID CONSTRUCTS USEFUL FOR GLUCOSE REGULATED PRODUCTION OF HUMAN INSULIN IN SOMATIC CELL LINES
有权
葡萄糖调控人胰岛素生产体细胞系SUITABLE NUCLEIC构建体
- 专利标题： NUCLEIC ACID CONSTRUCTS USEFUL FOR GLUCOSE REGULATED PRODUCTION OF HUMAN INSULIN IN SOMATIC CELL LINES
- 专利标题（中）： 葡萄糖调控人胰岛素生产体细胞系SUITABLE NUCLEIC构建体
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申请号： EP02741943.1申请日： 2002-06-10
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公开(公告)号： EP1458240A2公开(公告)日： 2004-09-22
- 发明人: TATAKE, Revati J.,Boehringer Ingelheim Corp. , SCHNEIDERMAN, Richard,Boehringer Ingelheim Corp. , BARTON, Randall Wilber,Boehringer Ingelheim, Corp
- 申请人： BOEHRINGER INGELHEIM PHARMACEUTICALS INC.
- 申请人地址： 900 Ridgebury Road,P.O. Box 368 Ridgefield,Connecticut 06877-0368 US
- 专利权人： BOEHRINGER INGELHEIM PHARMACEUTICALS INC.
- 当前专利权人： BOEHRINGER INGELHEIM PHARMACEUTICALS INC.
- 当前专利权人地址： 900 Ridgebury Road,P.O. Box 368 Ridgefield,Connecticut 06877-0368 US
- 代理机构： Mahlbacher, Volker
- 优先权： US296936P 20010608; US299334P 20010619
- 国际公布： WO2002101074 20021219
- 主分类号： A01N63/00
- IPC分类号： A01N63/00 ; A61K48/00 ; C12N15/00 ; C12N15/09 ; C12N15/63 ; C12N15/70 ; C12N15/74 ; C12N15/85 ; C12N15/87
摘要：
Unique multifunctional nucleotide expression cassettes comprising a nucleotide sequence encoding a human protease driven by a glucose-regulatable promoter, a nucleotide sequence encoding human proinsulin having sites at its B-C and C-A junctions altered to permit cleavage by the human protease, and a CMV promoter driving the transcription of the nucleotide sequence encoding the human proinsulin, such cassettes optionally having polyadenylation sequences after each structural gene and the nucleotide sequence encoding human proinsulin optionally carrying an H10D variant, and cells transformed thereby.
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