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    NOVEL PROMOTER VARIANT FOR CONSTITUTIVE EXPRESSION AND USE THEREOF
    2.
    发明公开
    NOVEL PROMOTER VARIANT FOR CONSTITUTIVE EXPRESSION AND USE THEREOF 审中-公开

    公开(公告)号：EP4438732A1

    公开(公告)日：2024-10-02

    申请号：EP23904796.2

    申请日：2023-04-07

    申请人： Daesang Corporation

    发明人： CHOI, Eun Seok ,  LEE, Ji Ha

    IPC分类号： C12N15/70 ,  C12N9/90 ,  C12P19/02

    CPC分类号： C12N9/90 ,  C12P19/02 ,  C12N15/70

    摘要： The present disclosure provides a novel promoter variant in which some nucleotides are inserted, deleted, or substituted in a twin arginine translocase A (tatA) gene promoter of Escherichia coli. According to the present disclosure, the novel promoter variant can constitutively high-express a target protein, particularly an enzyme, in Escherichia coli. Therefore, by using a recombinant strain transformed with an expression vector including the novel promoter variant according to the present disclosure, it is possible to economically mass-produce a target protein, particularly an enzyme. For example, by using a recombinant strain transformed with an expression vector including the novel promoter variant according to the present disclosure, it is possible to economically mass-produce allulose epimerase or economically mass-produce allulose from fructose.

    POLYPEPTIDE, FUSED POLYMER PROTEIN AND USE THEREOF
    9.
    发明公开
    POLYPEPTIDE, FUSED POLYMER PROTEIN AND USE THEREOF 审中-实审

    公开(公告)号：EP4353739A1

    公开(公告)日：2024-04-17

    申请号：EP22940501.4

    申请日：2022-12-21

    申请人： Sunresin New Materials Co., Ltd.

    发明人： LI, Yanjun ,  LI, Gang ,  GU, Tongnian ,  ZHANG, Jiantao ,  LIU, Long ,  LEI, Xiaoju ,  LIU, Qiong

    IPC分类号： C07K14/31 ,  C07K19/00 ,  C07K16/00 ,  C07K1/22 ,  C12N15/31 ,  C12N15/62 ,  C12N15/70 ,  C12N1/21 ,  G01N33/68 ,  B01D15/38 ,  C12R1/19

    摘要： The present invention relates to the technical field of protein biological functions, in particular to a polypeptide, a fusion type multimeric protein and use thereof, the polypeptide is selected from: (1), a polypeptide having a substitution mutation in at least one position selected from the group consisting of positions 16, 25, 29, 49 and 58, as compared with a natural C structural domain of a protein A as shown in SEQ ID NO.1; wherein, the position 16 is subjected to a substitution mutation into leucine or valine; the position 25 is subjected to a substitution mutation into lysine, arginine, histidine or tryptophan; the position 29 is subjected to a substitution mutation into alanine, leucine or threonine; the position 49 is subjected to a substitution mutation into arginine or histidine; and the position 58 is subjected to a substitution mutation into glycine, isoleucine or alanine; or (2) a polypeptide having at least 80% of sequence identity to the polypeptide in (1) and retaining substitution mutation in at least one position selected from the group consisting of positions 16, 25, 29, 49 and 58; the polypeptide has high alkali tolerance and high loading capacity.