The invention relates to the field of genetic engineering, more specifically to the field of production of glycoproteins, more specifically to the engineering of the glycosylation pattern of glycoproteins. Provided is a method for producing a glycoprotein in a recombinant host cell, comprising allowing expression of said glycoprotein in a basidiomycete host provided with N-acetylglucosamine : alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase (GnT-I; EC 2.4.1.101) activity. Also provided is a basidiomycete host cell expressing GnT-I, preferably human GnT-I and use thereof for the manufacture of (mammalian) proteins carrying complex N-glycans, such as (monoclonal) antibodies.