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公开(公告)号：EP1228231B1
公开(公告)日：2007-06-20
申请号：EP00980083.0
申请日：2000-10-26
CPC分类号： C12N9/1051 , C12N15/8257 , C12N15/8258
摘要： The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides a plant comprising a functional mammalian enzyme providing N-glycan biosynthesis that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.
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公开(公告)号：EP1228231A1
公开(公告)日：2002-08-07
申请号：EP00980083.0
申请日：2000-10-26
CPC分类号： C12N9/1051 , C12N15/8257 , C12N15/8258
摘要： The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides a plant comprising a functional mammalian enzyme providing N-glycan biosynthesis that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.
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3.发明公开
公开(公告)号：EP1673461A1
公开(公告)日：2006-06-28
申请号：EP04775003.9
申请日：2004-10-08
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/541 , A61K2039/55544 , C07K14/245 , C07K14/28 , C07K2319/00 , C12N15/8258 , C12N2770/24022 , Y02A50/472
摘要： The invention relates to a protein complex for the delivery of an antigen to and across mucosal surfaces and the production of said complex in a host cell, such as a plant. Provided is a protein complex comprising at least two, preferably identical, subunits wherein at least one subunit is unaltered and at least one subunit is fused to a first molecule of interest and wherein the protein complex is able to interact with a cell surface receptor via said subunits. Also provided is a method for producing a protein complex according to the invention, comprising a) providing a host cell with a nucleotide sequence encoding an unaltered subunit and a nucleotide sequence encoding a molecule of interest, wherein at least one molecule of interest is fused to a subunit; b) culturing said host cell thereby allowing expression of said nucleotide sequences and allowing for assembly of the protein complex; c) isolating the complex; d) determining the binding of the complex to a cell surface receptor or to a molecule which mimics a cell surface receptor.
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公开(公告)号：EP1228219B1
公开(公告)日：2005-11-09
申请号：EP00986056.0
申请日：2000-11-09
IPC分类号： C12N15/32 , C07K14/325 , C12N15/62 , A01H5/00 , A01N63/00
CPC分类号： C07K14/325 , C07K2319/00
摘要： Bacillus thuringiensis hybrid toxin fragment comprising structural domains I, II and III in this order starting from the N-terminal, wherein the domains are derived from at least two different Cry protein, domain I is domain I of any Bacillus thuringiensis Cry protein or a part of said domain or a peptide substantially similar to said domain, domain II is domain II or Cry 1 Ia or a part of said domain or a peptide substantially similar to said domain, and domain III is domain III of Cry1Ba or a part of said domain or a peptide substantially similar domain.
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5.发明公开GNTIII (UDP-N-ACETYLGLUCOSAMINE:BETA-D MANNOSIDE BETA(1,4)-N-ACETYLGLUCOSAMINYLTRANSFERASE III) EXPRESSION IN PLANTS 有权GNTIII（UDP-N-乙酰谷氨酰胺：BET-D-MANNOSID-BETA（1,4）-N-乙酰胆碱氨基转移酶III）PFLANZEN中的表达
公开(公告)号：EP1485492A2
公开(公告)日：2004-12-15
申请号：EP03715213.9
申请日：2003-03-18
发明人： BAKKER, Hendrikus, Antonius, Cornelus , FLORACK, Dionisius, Elisabeth, Antonius , BOSCH, Hendrik, Jan
IPC分类号： C12N15/82
CPC分类号： C12N15/8243 , C12N9/1051 , C12N15/8257 , C12P21/02
摘要： The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these glycoproteins. The invention provides a plant comprising a functional mammalian enzyme providing mammalian GnTII (EC 2.4.1.144)l that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.
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公开(公告)号：EP1861504B1
公开(公告)日：2009-12-16
申请号：EP06716639.7
申请日：2006-03-03
发明人： ROUWENDAL, Gerard, Johan, Adolph , FLORACK, Dionisius, Elisabeth, Antonius , BOSCH, Hendrik, Jan , LUGONES, Luis, Gast n
IPC分类号： C12P21/00
CPC分类号： C12P21/005
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7.发明公开
公开(公告)号：EP1266019A1
公开(公告)日：2002-12-18
申请号：EP01910240.9
申请日：2001-02-15
发明人： STEVENS, Lucas, Henricus , BOSCH, Hendrik, Jan , JORDI, Wilhelmus, Jacobus, Reinier, Marie , VERHOEVEN, Henricus, Adrianus
CPC分类号： C12N15/8249 , C12N15/8243 , C12N15/8257 , C12N15/8258
摘要： The invention relates to the field of the production and harvesting of plant products produced by biosynthesis in transgenic plants. The invention provides a method to increase the level of a desired product obtainable from a recombinant plant comprising allowing said plant to synthesise said product further comprising at least partly preventing degradation of said product.
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公开(公告)号：EP1861504A2
公开(公告)日：2007-12-05
申请号：EP06716639.7
申请日：2006-03-03
发明人： ROUWENDAL, Gerard, Johan, Adolph , FLORACK, Dionisius, Elisabeth, Antonius , BOSCH, Hendrik, Jan , LUGONES, Luis, Gast n
IPC分类号： C12P21/00
CPC分类号： C12P21/005
摘要： The invention relates to the field of genetic engineering, more specifically to the field of production of glycoproteins, more specifically to the engineering of the glycosylation pattern of glycoproteins. Provided is a method for producing a glycoprotein in a recombinant host cell, comprising allowing expression of said glycoprotein in a basidiomycete host provided with N-acetylglucosamine : alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase (GnT-I; EC 2.4.1.101) activity. Also provided is a basidiomycete host cell expressing GnT-I, preferably human GnT-I and use thereof for the manufacture of (mammalian) proteins carrying complex N-glycans, such as (monoclonal) antibodies.
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公开(公告)号：EP1485486A2
公开(公告)日：2004-12-15
申请号：EP03715229.5
申请日：2003-03-18
发明人： BAKKER, Hendrikus, Antonius, Cornelus , FLORACK, Dionisius, Elisabeth, Antonius , BOSCH, Hendrik, Jan , ROUWENDAL, Gerard, Johan, Adolph
CPC分类号： C12N9/1051 , C07K2317/13 , C07K2317/41 , C07K2319/02 , C12N15/8257 , C12N15/8258
摘要： The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus 5 containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.
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公开(公告)号：EP1228219A2
公开(公告)日：2002-08-07
申请号：EP00986056.0
申请日：2000-11-09
IPC分类号： C12N15/32 , C07K14/325 , C12N15/62 , A01H5/00 , A01N63/00
CPC分类号： C07K14/325 , C07K2319/00
摘要： Bacillus thuringiensis hybrid toxin fragment comprising structural domains I, II and III in this order starting from the N-terminal, wherein the domains are derived from at least two different Cry protein, domain I is domain I of any Bacillus thuringiensis Cry protein or a part of said domain or a peptide substantially similar to said domain, domain II is domain II or Cry 1 Ia or a part of said domain or a peptide substantially similar to said domain, and domain III is domain III of Cry1Ba or a part of said domain or a peptide substantially similar domain.
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