The present invention relates to food industry, particularly to microbiology and biotechnology of fermented milk products, and may be applied to production of starter (dedo) for "matsoni", wholesome and innocuous Georgian fermented milk product. The applied method for producing and storage of starter for a yogurt-like fermented milk product matsoni, implies an inclusion of a symbiotic combination of at least two kinds y of bacteria, Lactobacillus delbrueckii subsp. lactis and Streptococcus thermofilus in its composition, inoculation and incubation of them in milk medium, wherein the amounts of their cells are taken within the ratio 1/ (2oe). The symbiotic bacterial culture of Lactobacillus delbrueckii subsp. lactis and Streptococcus thermofilus is stored in lyophilic form until being inoculated in milk for processing matsoni.