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EP2258846A2 A method for increasing the concentration of a nucleic acid molecule
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Verfahren zurErhöhungder Konzentration einesNucleinsäuremoleküls
- 专利标题： A method for increasing the concentration of a nucleic acid molecule
- 专利标题（中）： Verfahren zurErhöhungder Konzentration einesNucleinsäuremoleküls
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申请号： EP10184602.0申请日： 1998-06-29
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公开(公告)号： EP2258846A2公开(公告)日： 2010-12-08
- 发明人: Griffiths, Andrew , Tawfik, Dan
- 申请人： MEDICAL RESEARCH COUNCIL
- 申请人地址： 20 Park Crescent London W1B 1AL GB
- 专利权人： MEDICAL RESEARCH COUNCIL
- 当前专利权人： MEDICAL RESEARCH COUNCIL
- 当前专利权人地址： 20 Park Crescent London W1B 1AL GB
- 代理机构： Finnie, Isobel Lara
- 优先权： GB9714300 19970707; GB9806393 19980325
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12Q1/68
摘要：
A method for increasing the concentration of a nucleic acid molecule, said method comprising:
(a) forming aqueous microcapsules from a water-in-oil emulsion, wherein a plurality of the microcapsules include a nucleic acid molecule and an aqueous solution comprising components necessary for nucleic acid amplification; and
(b) amplifying the nucleic acid molecule in the microcapsules to form further amplified copies of said nucleic acid molecule.
(a) forming aqueous microcapsules from a water-in-oil emulsion, wherein a plurality of the microcapsules include a nucleic acid molecule and an aqueous solution comprising components necessary for nucleic acid amplification; and
(b) amplifying the nucleic acid molecule in the microcapsules to form further amplified copies of said nucleic acid molecule.
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