发明公开
EP2373340A2 RANDOM HOMOZYGOUS GENE PERBURBATION (RHGP) WITH THERMAL ASSYMETRIC INTERLACED (TAIL)-PCR
审中-公开
随机同源基因灌木（RHGP）与热协方差交错（尾）-PCR
- 专利标题： RANDOM HOMOZYGOUS GENE PERBURBATION (RHGP) WITH THERMAL ASSYMETRIC INTERLACED (TAIL)-PCR
- 专利标题（中）： 随机同源基因灌木（RHGP）与热协方差交错（尾）-PCR
-
申请号： EP10729431.6申请日： 2010-01-06
-
公开(公告)号： EP2373340A2公开(公告)日： 2011-10-12
- 发明人: KINCH, Michael, S. , GOLDBLATT, Michael , LI, Wu-Bo , BAMBA, Douty , CHANG, Shaojing , CHEN, Huosheng , FESSEHA, Zenbework , KOHLI, Manu , MAO, Hanwen , UNG-MEDOFF, Heather Thi Thu , WENG, Ke
- 申请人： Functional Genetics, Inc.
- 申请人地址： 708 Quince Orchard Road Suite 200 Gaithersburg, Maryland 20878 US
- 专利权人： Functional Genetics, Inc.
- 当前专利权人： Functional Genetics, Inc.
- 当前专利权人地址： 708 Quince Orchard Road Suite 200 Gaithersburg, Maryland 20878 US
- 代理机构： Sexton, Jane Helen
- 优先权： US142664P 20090106; US153012P 20090217; US158015P 20090306; US160737P 20090317; US178112P 20090514; US180457P 20090522
- 国际公布： WO2010080782 20100715
- 主分类号： A61K39/395
- IPC分类号： A61K39/395 ; C12Q1/70 ; A61P31/16 ; A61P31/18
摘要：
A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL) - PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.
信息查询
